Measuring free ellagic acid: influence of extraction conditions on recovery by studying solubility and UV-Visible spectra

Williams, David J., Edwards, David, Chaliha, Mridusmita and Sultanbawa, Yasmina (2016) Measuring free ellagic acid: influence of extraction conditions on recovery by studying solubility and UV-Visible spectra. Chemical Papers, 70 8: 1078-1086. doi:10.1515/chempap-2016-0038


Author Williams, David J.
Edwards, David
Chaliha, Mridusmita
Sultanbawa, Yasmina
Title Measuring free ellagic acid: influence of extraction conditions on recovery by studying solubility and UV-Visible spectra
Journal name Chemical Papers   Check publisher's open access policy
ISSN 0366-6352
1336-9075
Publication date 2016-04-14
Sub-type Article (original research)
DOI 10.1515/chempap-2016-0038
Open Access Status Not Open Access
Volume 70
Issue 8
Start page 1078
End page 1086
Total pages 9
Place of publication Bratislava, Slovakia
Publisher Slovenska Akademia Vied * Chemicky Ustav
Language eng
Formatted abstract
Recent investigations into plant tissues have indicated that the free form of the natural polyphenolic antioxidant, ellagic acid (EA), is much more plentiful than first envisaged; consequently a re-assessment of solvent systems for the extraction of this water-insoluble form is needed. As EA solubility and its UV-Vis spectrum, commonly used for detection and quantification, are both governed by pH, an understanding of this dependence is vital if accurate EA measurements are to be achieved. After evaluating the pH effects on the solubility and UV-Vis spectra of commercial EA, an extraction protocol was devised that promoted similar pH conditions for both standard solutions and plant tissue extracts. The extraction so devised followed by HPLC with photodiode-array detection (DAD) provided a simple, sensitive and validated methodology that determined free EA in a variety of plant extracts. The use of 100 % methanol or a triethanolamine-based mixture as the standard dissolving solvents were the best choices, while these higher pH-generating solvents were more efficient in extracting EA from the plants tested with the final choice allied to the plants’ natural acidity. Two of the native Australian plants anise myrtle (Syzygium anisatum) and Kakadu plum (Terminalia ferdinandiana) exhibited high concentrations of free EA. Furthermore, the dual approach to measuring EA UV-Vis spectra made possible an assessment of the effect of acidified eluent on EA spectra when the DAD was employed.
Keyword Ellagic acid
Solubility
pH
UV-Vis spectra
Australian native plants
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Centre for Nutrition and Food Sciences Publications
 
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Created: Thu, 12 May 2016, 07:54:16 EST by Dr Yasmina Sultanbawa on behalf of Centre for Nutrition and Food Sciences