Electrochemical detection of protein glycosylation using lectin and protein-gold affinity interactions

Yadav, Sharda, Carrascosa, Laura G., Sina, Abu A. I., Shiddiky, Muhammad J. A., Hill, Michelle M. and Trau, Matt (2016) Electrochemical detection of protein glycosylation using lectin and protein-gold affinity interactions. Analyst, 141 8: 2356-2361. doi:10.1039/c6an00528d


Author Yadav, Sharda
Carrascosa, Laura G.
Sina, Abu A. I.
Shiddiky, Muhammad J. A.
Hill, Michelle M.
Trau, Matt
Title Electrochemical detection of protein glycosylation using lectin and protein-gold affinity interactions
Journal name Analyst   Check publisher's open access policy
ISSN 0003-2654
1364-5528
Publication date 2016-03-11
Year available 2016
Sub-type Article (original research)
DOI 10.1039/c6an00528d
Open Access Status Not Open Access
Volume 141
Issue 8
Start page 2356
End page 2361
Total pages 6
Place of publication Cambridge, United Kingdom
Publisher Royal Society of Chemistry
Language eng
Formatted abstract
We report a new method for the electrochemical detection of glycosylation on proteins, which relies on lectin–protein interaction on a bare gold electrode. The target protein isolated by immunoaffinity is directly adsorbed onto a gold surface and its glycosylation status is retrieved by subsequent addition of specific lectins. The adsorption and subsequent recognition process is monitored electrochemically in the presence of [Fe(CN)6]3−/4− redox system. By decoupling target protein capture from glycosylation read-out steps, this approach circumvents unwanted antibody–lectin crosstalk while enabling specific glycosylation detection of a glycoprotein in serum-spiked samples in less than 1 h.
Keyword Yeast-Scfv Probes
Label-Free
Mass-Spectrometry
Dna Methylation
Cancer
Biomarkers
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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