Mechanisms involved in acquisition of blaNDM genes by IncA/C2 and IncFIIY plasmids

Wailan, Alexander M., Sidjabat, Hanna E., Yam, Wan Keat, Alikhan, Nabil-Fareed, Petty, Nicola K., Sartor, Anna L., Williamson, Deborah A., Forde, Brian M., Schembri, Mark A., Beatson, Scott A., Paterson, David L., Walsh, Timothy R. and Partridge, Sally R. (2016) Mechanisms involved in acquisition of blaNDM genes by IncA/C2 and IncFIIY plasmids. Antimicrobial Agents and Chemotherapy, 60 7: 4082-4088. doi:10.1128/AAC.00368-16

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Author Wailan, Alexander M.
Sidjabat, Hanna E.
Yam, Wan Keat
Alikhan, Nabil-Fareed
Petty, Nicola K.
Sartor, Anna L.
Williamson, Deborah A.
Forde, Brian M.
Schembri, Mark A.
Beatson, Scott A.
Paterson, David L.
Walsh, Timothy R.
Partridge, Sally R.
Title Mechanisms involved in acquisition of blaNDM genes by IncA/C2 and IncFIIY plasmids
Formatted title
Mechanisms involved in acquisition of blaNDM genes by IncA/C2 and IncFIIY plasmids
Journal name Antimicrobial Agents and Chemotherapy   Check publisher's open access policy
ISSN 1098-6596
Publication date 2016-04-25
Year available 2016
Sub-type Article (original research)
DOI 10.1128/AAC.00368-16
Open Access Status File (Author Post-print)
Volume 60
Issue 7
Start page 4082
End page 4088
Total pages 26
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Subject 3004 Pharmacology
2736 Pharmacology (medical)
2725 Infectious Diseases
Abstract blaNDM genes confer carbapenem resistance and have been identified on transferable plasmids belonging to different incompatibility (Inc) groups. Here we present the complete sequences of four plasmids carrying a blaNDM gene, pKP1-NDM-1, pEC2-NDM-3, pECL3-NDM-1, and pEC4-NDM-6, from four clinical samples originating from four different patients. Different plasmids carry segments that align to different parts of the blaNDM region found on Acinetobacter plasmids. pKP1-NDM-1 and pEC2-NDM-3, from Klebsiella pneumoniae and Escherichia coli, respectively, were identified as type 1 IncA/C2 plasmids with almost identical backbones. Different regions carrying blaNDM are inserted in different locations in the antibiotic resistance island known as ARI-A, and ISCR1 may have been involved in the acquisition of blaNDM-3 by pEC2-NDM-3. pECL3-NDM-1 and pEC4-NDM-6, from Enterobacter cloacae and E. coli, respectively, have similar IncFIIY backbones, but different regions carrying blaNDM are found in different locations. Tn3-derived inverted-repeat transposable elements (TIME) appear to have been involved in the acquisition of blaNDM-6 by pEC4-NDM-6 and the rmtC 16S rRNA methylase gene by IncFIIY plasmids. Characterization of these plasmids further demonstrates that even very closely related plasmids may have acquired blaNDM genes by different mechanisms. These findings also illustrate the complex relationships between antimicrobial resistance genes, transposable elements, and plasmids and provide insights into the possible routes for transmission of blaNDM genes among species of the Enterobacteriaceae family.
Keyword Microbiology
Pharmacology & Pharmacy
Pharmacology & Pharmacy
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID GNT1106930
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
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School of Chemistry and Molecular Biosciences
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Citation counts: TR Web of Science Citation Count  Cited 9 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 8 times in Scopus Article | Citations
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Created: Fri, 06 May 2016, 19:58:32 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences