The transcriptomic response of the coral Acropora digitifera to a competent Symbiodinium strain: the symbiosome as an arrested early phagosome

Mohamed, A. R., Cumbo, V., Harii, S., Shinzato,C., Chan, C. X., Ragan, M. A., Bourne, D. G., Willis, B. L., Ball, E. E., Satoh, N. and Miller, D. J. (2016) The transcriptomic response of the coral Acropora digitifera to a competent Symbiodinium strain: the symbiosome as an arrested early phagosome. Molecular Ecology, 25 13: 3127-3141. doi:10.1111/mec.13659


Author Mohamed, A. R.
Cumbo, V.
Harii, S.
Shinzato,C.
Chan, C. X.
Ragan, M. A.
Bourne, D. G.
Willis, B. L.
Ball, E. E.
Satoh, N.
Miller, D. J.
Title The transcriptomic response of the coral Acropora digitifera to a competent Symbiodinium strain: the symbiosome as an arrested early phagosome
Formatted title
The transcriptomic response of the coral Acropora digitifera to a competent Symbiodinium strain: the symbiosome as an arrested early phagosome
Journal name Molecular Ecology   Check publisher's open access policy
ISSN 0962-1083
1365-294X
Publication date 2016-07-01
Year available 2016
Sub-type Article (original research)
DOI 10.1111/mec.13659
Open Access Status DOI
Volume 25
Issue 13
Start page 3127
End page 3141
Total pages 15
Place of publication West Sussex, United Kingdom
Publisher Wiley-Blackwell
Language eng
Subject 1105 Ecology, Evolution, Behavior and Systematics
1311 Genetics
Abstract Despite the ecological significance of the relationship between reef-building corals and intracellular photosynthetic dinoflagellates of the genus Symbiodinium, very little is known about the molecular mechanisms involved in its establishment. Indeed, microarray-based analyses point to the conclusion that host gene expression is largely or completely unresponsive during the establishment of symbiosis with a competent strain of Symbiodinium. In this study, the use of Illumina RNA-Seq technology allowed detection of a transient period of differential expression involving a small number of genes (1073 transcripts; <3% of the transcriptome) 4 h after the exposure of Acropora digitifera planulae to a competent strain of Symbiodinium (a clade B strain). This phenomenon has not previously been detected as a consequence of both the lower sensitivity of the microarray approaches used and the sampling times used. The results indicate that complex changes occur, including transient suppression of mitochondrial metabolism and protein synthesis, but are also consistent with the hypothesis that the symbiosome is a phagosome that has undergone early arrest, raising the possibility of common mechanisms in the symbiotic interactions of corals and symbiotic sea anemones with their endosymbionts.
Formatted abstract
Despite the ecological significance of the relationship between reef-building coralsand intracellular photosynthetic dinoflagellates of the genus Symbiodinium, very littleis known about the molecular mechanisms involved in its establishment. Indeed,microarray-based analyses point to the conclusion that host gene expression is largelyor completely unresponsive during the establishment of symbiosis with a competentstrain of Symbiodinium. In this study, the use of Illumina RNA-Seq technologyallowed detection of a transient period of differential expression involving a smallnumber of genes (1073 transcripts; <3% of the transcriptome) 4 h after the exposure of Acropora digitifera planulae to a competent strain of Symbiodinium (a clade B strain).This phenomenon has not previously been detected as a consequence of both thelower sensitivity of the microarray approaches used and the sampling times used. The results indicate that complex changes occur, including transient suppression of mito-chondrial metabolism and protein synthesis, but are also consistent with the hypothesisthat the symbiosome is a phagosome that has undergone early arrest, raising the possi-bility of common mechanisms in the symbiotic interactions of corals and symbiotic seaanemones with their endosymbionts.
Keyword Biochemistry & Molecular Biology
Ecology
Evolutionary Biology
Biochemistry & Molecular Biology
Environmental Sciences & Ecology
Evolutionary Biology
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID CE140100020
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Institute for Molecular Bioscience - Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 13 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 15 times in Scopus Article | Citations
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Created: Fri, 06 May 2016, 01:56:11 EST by Susan Allen on behalf of Institute for Molecular Bioscience