A new non-degradative method to purify glycogen

Tan, Xinle, Sullivan, Mitchell A., Gao, Fei, Li, Shihan, Schulz, Benjamin L. and Gilbert, Robert G. (2016) A new non-degradative method to purify glycogen. Carbohydrate Polymers, 147 165-170. doi:10.1016/j.carbpol.2016.04.009

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Author Tan, Xinle
Sullivan, Mitchell A.
Gao, Fei
Li, Shihan
Schulz, Benjamin L.
Gilbert, Robert G.
Title A new non-degradative method to purify glycogen
Journal name Carbohydrate Polymers   Check publisher's open access policy
ISSN 0144-8617
1879-1344
Publication date 2016-08-20
Year available 2016
Sub-type Article (original research)
DOI 10.1016/j.carbpol.2016.04.009
Open Access Status File (Author Post-print)
Volume 147
Start page 165
End page 170
Total pages 6
Place of publication Kidlington, Oxford, United Kingdom
Publisher Pergamon Press
Language eng
Subject 2507 Polymers and Plastics
1605 Organic Chemistry
2505 Materials Chemistry
Abstract Liver glycogen, a complex branched glucose polymer containing a small amount of protein, is important for maintaining glucose homeostasis (blood-sugar control) in humans. It has recently been found that glycogen molecular structure is impaired in diabetes. Isolating the carbohydrate polymer and any intrinsically-attached protein(s) is an essential prerequisite for studying this structural impairment. This requires an effective, non-degradative and efficient purification method to exclude the many other proteins present in liver. Proteins and glycogen have different ranges of molecular sizes. Despite the plethora of proteins that might still be present in significant abundance after other isolation techniques, SEC (size exclusion chromatography, also known as GPC), which separates by molecular size, should separate those extraneous to glycogen from glycogen with any intrinsically associated protein(s). A novel purification method is developed for this, based on preparative SEC following sucrose gradient centrifugation. Proteomics is used to show that the new method compares favourably with current methods in the literature.
Keyword Glycogen
GPC
Mass spectrometry
Protein
SEC
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID Y15G-GILR
APP1087975
GNT1092451
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Queensland Alliance for Agriculture and Food Innovation
School of Chemistry and Molecular Biosciences
 
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