Mesenchymal cells appearing in pancreatic tissue culture are bone marrow-derived stem cells with the capacity to improve transplanted islet function

Sordi, Valeria, Melzi, Raffaella, Mercalli, Alessia, Formicola, Roberta, Doglioni, Claudio, Tiboni, Francesca, Ferrari, Giuliana, Nano, Rita, Chwalek, Karolina, Lammert, Eckhard, Bonifacio, Ezio, Borg, Danielle and Piemonti, Lorenzo (2010) Mesenchymal cells appearing in pancreatic tissue culture are bone marrow-derived stem cells with the capacity to improve transplanted islet function. Stem Cells, 28 1: 140-151. doi:10.1002/stem.259


Author Sordi, Valeria
Melzi, Raffaella
Mercalli, Alessia
Formicola, Roberta
Doglioni, Claudio
Tiboni, Francesca
Ferrari, Giuliana
Nano, Rita
Chwalek, Karolina
Lammert, Eckhard
Bonifacio, Ezio
Borg, Danielle
Piemonti, Lorenzo
Title Mesenchymal cells appearing in pancreatic tissue culture are bone marrow-derived stem cells with the capacity to improve transplanted islet function
Journal name Stem Cells   Check publisher's open access policy
ISSN 1066-5099
1549-4918
Publication date 2010-01-01
Sub-type Article (original research)
DOI 10.1002/stem.259
Open Access Status Not yet assessed
Volume 28
Issue 1
Start page 140
End page 151
Total pages 12
Place of publication Durham, NC, United States
Publisher AlphaMed Press
Language eng
Abstract Adherent fibroblast-like cells have been reported to appear in cultures of human endocrine or exocrine pancreatic tissue during attempts to differentiate human β cells from pancreatic precursors. A thorough characterization of these mesenchymal cells has not yet been completed, and there are no conclusive data about their origin. We demonstrated that the human mesenchymal cells outgrowing from cultured human pancreatic endocrine or exocrine tissue are pancreatic mesenchymal stem cells (pMSC) that propagate from contaminating pMSC. The origin of pMSC is partly extrapancreatic both in humans and mice, and by using green fluorescent protein (GFP+) bone marrow transplantation in the mouse model, we were able to demonstrate that these cells derive from the CD45+ component of bone marrow. The pMSC express negligible levels of islet-specific genes both in basal conditions and after serum deprivation or exogenous growth factor exposure, and might not represent optimal candidates for generation of physiologically competent β-cells. On the other hand, when cotransplanted with a minimal pancreatic islet mass, pMSC facilitate the restoration of normoglycemia and the neovascularization of the graft. These results suggest that pMSCs could exert an indirect role of "helper" cells in tissue repair processes.
Keyword Islet function
Mesenchymal cells
Pancreatic tissue
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Mater Research Institute-UQ (MRI-UQ)
 
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