Liposome-based intranasal Delivery of lipopeptide vaccine candidates against Group A Streptococcus

Ghaffar, Khairunnisa Abdul, Marasini, Nirmal, Giddam, Ashwini K., Batzloff, Michael, Good, Michael, Skwarczynski, Mariusz and Toth, Istvan (2016) Liposome-based intranasal Delivery of lipopeptide vaccine candidates against Group A Streptococcus. Acta Biomaterialia, 41 161-168. doi:10.1016/j.actbio.2016.04.012


Author Ghaffar, Khairunnisa Abdul
Marasini, Nirmal
Giddam, Ashwini K.
Batzloff, Michael
Good, Michael
Skwarczynski, Mariusz
Toth, Istvan
Title Liposome-based intranasal Delivery of lipopeptide vaccine candidates against Group A Streptococcus
Journal name Acta Biomaterialia   Check publisher's open access policy
ISSN 1742-7061
1878-7568
Publication date 2016-04-07
Year available 2016
Sub-type Article (original research)
DOI 10.1016/j.actbio.2016.04.012
Open Access Status Not Open Access
Volume 41
Start page 161
End page 168
Total pages 34
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Abstract Group A streptococcus (GAS), an exclusively human pathogen, causes a wide range of diseases ranging from trivial to life threatening. Treatment of infection is often ineffective following entry of bacteria into the bloodstream. To date, there is no vaccine available against GAS. In this study, cationic liposomes encapsulating lipopeptide-based vaccine candidates against GAS have been employed for intranasal vaccine delivery. Cationic liposomes were prepared with dimethyldioctadecylammonium bromide (DDAB) using the film hydration method. Female Swiss mice were immunized intranasally with the liposomes. In contrast to unmodified peptides, lipopeptides entrapped by liposomes induced both mucosal and systemic immunity, IgA and IgG (IgG1 and IgG2a) production in mice, respectively. High levels of antibody (IgA and IgG) titres were detected even five months post immunization. Thus, the combination of lipopeptides and liposomes generates a very promising delivery system for intranasal vaccines.

Group A streptococcus, causing rheumatic heart diseases, kills approximately half a million people annually. There is no vaccine available against the infection. Mucosal immunity is vital in ensuring an individual is protected as this gram positive bacteria initially colonizes at the throat. Herein, we demonstrated that lipopeptides entrapped by liposomes induced both mucosal and systemic immunity. High levels of antibody (IgA and IgG) titres were detected even five months post immunization and lead vaccine candidate was able to induce humoral immune responses even after single immunization. Thus, the combination of lipopeptides and liposomes generates a very promising delivery system for intranasal vaccines.
Keyword Cationic liposomes
Group A Streptococcus
Intranasal immunization
Peptide vaccine
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 8 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 8 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Fri, 15 Apr 2016, 23:45:40 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences