Direct electrochemistry of nitrate reductase from the fungus Neurospora crassa

Kalimuthu, Palraj, Ringel, Phillip, Kruse, Tobias and Bernhardt, Paul V. (2016) Direct electrochemistry of nitrate reductase from the fungus Neurospora crassa. Biochimica et Biophysica Acta. Bioenergetics, 1857 9: 1506-1513. doi:10.1016/j.bbabio.2016.04.001

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Author Kalimuthu, Palraj
Ringel, Phillip
Kruse, Tobias
Bernhardt, Paul V.
Title Direct electrochemistry of nitrate reductase from the fungus Neurospora crassa
Formatted title
Direct electrochemistry of nitrate reductase from the fungus Neurospora crassa
Journal name Biochimica et Biophysica Acta. Bioenergetics   Check publisher's open access policy
ISSN 0005-2728
Publication date 2016-04-07
Sub-type Article (original research)
DOI 10.1016/j.bbabio.2016.04.001
Open Access Status File (Author Post-print)
Volume 1857
Issue 9
Start page 1506
End page 1513
Total pages 23
Place of publication Amsterdam, Netherlands
Publisher Elsevier
Language eng
Subject 1304 Biophysics
1303 Biochemistry
1307 Cell Biology
Abstract We report the first direct (unmediated) catalytic electrochemistry of a eukaryotic nitrate reductase (NR). NR from the filamentous fungus Neurospora crassa, is a member of the mononuclear molybdenum enzyme family and contains a Mo, heme and FAD cofactor which are involved in electron transfer from NAD(P)H to the (Mo) active site where reduction of nitrate to nitrite takes place. NR was adsorbed on an edge plane pyrolytic graphite (EPG) working electrode. Non-turnover redox responses were observed in the absence of nitrate from holo NR and three variants lacking the FAD, heme or Mo cofactor. The FAD response is due to dissociated cofactor in all cases. In the presence of nitrate, NR shows a pronounced cathodic catalytic wave with an apparent Michaelis constant (K) of 39 μM (pH 7). The catalytic cathodic current increases with temperature from 5 to 35 °C and an activation enthalpy of 26 kJ mol was determined. In spite of dissociation of the FAD cofactor, catalytically activity is maintained.
Keyword Enzyme
Molybdenum
Nitrate reductase
Voltammetry
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID DP150103345
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Chemistry and Molecular Biosciences
 
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Created: Fri, 15 Apr 2016, 23:38:24 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences