Neogenin recruitment of the WAVE regulatory complex maintains adherens junction stability and tension

Lee, Natalie K., Fok, Ka Wai, White, Amanda, Wilson, Nicole H., O’Leary, Conor J., Cox, Hayley L., Michael, Magdalene, Yap, Alpha S. and Cooper, Helen M. (2016) Neogenin recruitment of the WAVE regulatory complex maintains adherens junction stability and tension. Nature Communications, 7 11082: . doi:10.1038/ncomms11082

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Author Lee, Natalie K.
Fok, Ka Wai
White, Amanda
Wilson, Nicole H.
O’Leary, Conor J.
Cox, Hayley L.
Michael, Magdalene
Yap, Alpha S.
Cooper, Helen M.
Title Neogenin recruitment of the WAVE regulatory complex maintains adherens junction stability and tension
Journal name Nature Communications   Check publisher's open access policy
ISSN 2041-1723
Publication date 2016-03-31
Sub-type Article (original research)
DOI 10.1038/ncomms11082
Open Access Status DOI
Volume 7
Issue 11082
Total pages 13
Place of publication London, United Kingdom
Publisher Nature Publishing Group
Language eng
Formatted abstract
To maintain tissue integrity during epithelial morphogenesis, adherens junctions (AJs) must resist the mechanical stresses exerted by dynamic tissue movements. Junctional stability is dependent on actomyosin contractility within the actin ring. Here we describe a novel function for the axon guidance receptor, Neogenin, as a key component of the actin nucleation machinery governing junctional stability. Loss of Neogenin perturbs AJs and attenuates junctional tension. Neogenin promotes actin nucleation at AJs by recruiting the Wave regulatory complex (WRC) and Arp2/3. A direct interaction between the Neogenin WIRS domain and the WRC is crucial for the spatially restricted recruitment of the WRC to the junction. Thus, we provide the first example of a functional WIRS–WRC interaction in epithelia. We further show that Neogenin regulates cadherin recycling at the AJ. In summary, we identify Neogenin as a pivotal component of the AJ, where it influences both cadherin dynamics and junctional tension.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Queensland Brain Institute Publications
Institute for Molecular Bioscience - Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 3 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 3 times in Scopus Article | Citations
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Created: Thu, 07 Apr 2016, 03:35:15 EST by Conor O'leary on behalf of Queensland Brain Institute