Human mesenchymal stem cells alter the gene profile of monocytes from patients with Type 2 diabetes and end-stage renal disease

Wise, Andrea F., Williams, Timothy M., Rudd, Stephen, Wells, Christine A., Kerr, Peter G. and Ricardo, Sharon D. (2016) Human mesenchymal stem cells alter the gene profile of monocytes from patients with Type 2 diabetes and end-stage renal disease. Regenerative Medicine, 11 2: 145-158. doi:10.2217/rme.15.74


Author Wise, Andrea F.
Williams, Timothy M.
Rudd, Stephen
Wells, Christine A.
Kerr, Peter G.
Ricardo, Sharon D.
Title Human mesenchymal stem cells alter the gene profile of monocytes from patients with Type 2 diabetes and end-stage renal disease
Journal name Regenerative Medicine   Check publisher's open access policy
ISSN 1746-0751
1746-076X
Publication date 2016-03-01
Sub-type Article (original research)
DOI 10.2217/rme.15.74
Open Access Status Not Open Access
Volume 11
Issue 2
Start page 145
End page 158
Total pages 14
Place of publication London, United Kingdom
Publisher Future Medicine
Language eng
Formatted abstract
Aim: Macrophage infiltration contributes to the pathogenesis of Type 2 diabetes. Mesenchymal stem cells (MSCs) possess immunomodulatory properties, making them an ideal candidate for therapeutic intervention. This study investigated whether MSCs can modulate the phenotype of monocytes isolated from Type 2 diabetic patients with end-stage renal disease.

Materials & methods: Monocytes from control (n = 4) and Type 2 diabetic patients with end-stage renal disease (n = 5) were assessed using flow cytometry and microarray profiling, following 48 h of co-culture with MSCs.

Results: Control subjects had a greater proportion of CD14++CD16- monocytes while diabetic patients had a higher proportion of CD14++CD16+ and CD14+CD16++ monocytes. MSCs promoted the proliferation of monocytes isolated from diabetic patients, reduced HLA-DR expression in both groups and promoted the expression of anti-inflammatory genes.

Conclusion: MSC-derived factors alter the polarization of monocytes isolated from healthy and diabetic subjects toward an M2 phenotype.
Keyword Type 2 diabetes
Ischemia-Reperfusion Injury
Stromal Cells
Bone-Marrow
Insulin-Resistance
Dendritic Cells
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

 
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