Effect of activated protein C in second intention healing of equine distal limb wounds: a preliminary study

Bischofberger, A. S., Tsang, A. S., Horadagoda, N., Dart, C. M., Perkins, N. R., Jeffcott, L. B., Jackson, C. J. and Dart, A. J. (2015) Effect of activated protein C in second intention healing of equine distal limb wounds: a preliminary study. Australian Veterinary Journal, 93 10: 361-366. doi:10.1111/avj.12363


Author Bischofberger, A. S.
Tsang, A. S.
Horadagoda, N.
Dart, C. M.
Perkins, N. R.
Jeffcott, L. B.
Jackson, C. J.
Dart, A. J.
Title Effect of activated protein C in second intention healing of equine distal limb wounds: a preliminary study
Journal name Australian Veterinary Journal   Check publisher's open access policy
ISSN 0005-0423
1751-0813
Publication date 2015-10-28
Year available 2015
Sub-type Article (original research)
DOI 10.1111/avj.12363
Open Access Status Not Open Access
Volume 93
Issue 10
Start page 361
End page 366
Total pages 6
Place of publication Chichester, West Sussex United Kingdom
Publisher Wiley-Blackwell Publishing
Language eng
Formatted abstract
Objective
To investigate the effect of activated protein C (APC) on second intention healing of distal limb wounds in horses.

Methods
In this experimental study of eight Standardbred geldings, six full-thickness skin wounds (2 × 1.5 cm) were created on one metacarpus (biopsy limb) and five similar wounds were created on the contralateral metacarpus (photographed limb). Three wounds on the biopsy limb were treated topically with 190 µg APC on days 1, 3, 6 and 9, while the remaining three wounds were untreated (control). One treated and one control wound were biopsied on days 4, 7 and 11 for histopathology. Wounds on the photographed limb were treated with either 66% Manuka honey gel, a commercial antibiotic ointment (bacitracin–neomycin–polymixin B ointment; BNP) or petrolatum daily throughout healing, treated on days 1,3,6 and 9 with 190 µg APC or left untreated. These wounds were digitally photographed and the wound area measured on day 1, then weekly until day 49. Overall time to healing was recorded.

Results
There was no effect of APC on wound size, the rate of healing or the overall time to heal. However, compared with control wounds, histological scoring demonstrated enhanced epithelialisation (day 4) and angiogenesis (day 11). Wound healing variables for wounds treated with APC, Manuka honey gel and control wounds were not different and the variables for wounds treated with BNP and petrolatum demonstrated delayed healing.

Conclusion
The improvements in histological scores in APC-treated wounds suggest further study into the effect of APC on second intention wound healing in horses is warranted.
Keyword Activated protein C
Bacitracin
Horses
Manuka honey gel
Neomycin
Polymixin B ointment
Wound healing
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
School of Veterinary Science Publications
 
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Created: Thu, 31 Mar 2016, 00:50:43 EST by Nigel Perkins on behalf of School of Veterinary Science