Synthesis of 19F nucleic acid-polymer conjugates as real-time MRI probes of biorecognition

Sicilia, Giovanna, Davis, Adrienne L., Spain, Sebastian G., Magnusson, Johannes P., Boase, Nathan R. B., Thurecht, Kristofer J. and Alexander, Cameron (2016) Synthesis of 19F nucleic acid-polymer conjugates as real-time MRI probes of biorecognition. Polymer Chemistry, 7 12: 2180-2191. doi:10.1039/c5py01883h


Author Sicilia, Giovanna
Davis, Adrienne L.
Spain, Sebastian G.
Magnusson, Johannes P.
Boase, Nathan R. B.
Thurecht, Kristofer J.
Alexander, Cameron
Title Synthesis of 19F nucleic acid-polymer conjugates as real-time MRI probes of biorecognition
Formatted title
Synthesis of 19F nucleic acid-polymer conjugates as real-time MRI probes of biorecognition
Journal name Polymer Chemistry   Check publisher's open access policy
ISSN 1759-9962
1759-9954
Publication date 2016-03-28
Year available 2016
Sub-type Article (original research)
DOI 10.1039/c5py01883h
Open Access Status Not Open Access
Volume 7
Issue 12
Start page 2180
End page 2191
Total pages 12
Place of publication Cambridge, United Kingdom
Publisher Royal Society of Chemistry
Language eng
Formatted abstract
Polymer–DNA conjugates in which one nucleic acid strand contains fluorine-substituted nucleobases have been prepared and characterised. The efficacy of these novel 19F nucleic acid–polymer conjugates as sensitive and selective in vitro reporters of DNA binding events is demonstrated through a number of rapid-acquisition MR sequences. The conjugates respond readily and in a sequence specific manner to external target oligonucleotide sequences by changes in hybridisation. In turn, these structural changes in polymer–nucleotide conjugates translate into responses which are detectable in fluorine relaxation and diffusion switches, and which can be monitored by in vitro Spin Echo and DOSY NMR spectroscopy. Although complementary to conventional FRET methods, the excellent diagnostic properties of fluorine nuclei make this approach a versatile and sensitive probe of molecular structure and conformation in polymeric assemblies.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Australian Institute for Bioengineering and Nanotechnology Publications
Centre for Advanced Imaging Publications
 
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