Simultaneous quantitative analysis of nine vitamin D compounds in human blood using LC-MS/MS

Abu Kassim, Nur Sofiah, Gomes, Fabio P., Shaw, Paul Nicholas and Hewavitharana, Amitha K. (2016) Simultaneous quantitative analysis of nine vitamin D compounds in human blood using LC-MS/MS. Bioanalysis, 8 5: 397-411. doi:10.4155/bio.15.260


Author Abu Kassim, Nur Sofiah
Gomes, Fabio P.
Shaw, Paul Nicholas
Hewavitharana, Amitha K.
Title Simultaneous quantitative analysis of nine vitamin D compounds in human blood using LC-MS/MS
Journal name Bioanalysis   Check publisher's open access policy
ISSN 1757-6180
1757-6199
Publication date 2016-03-01
Year available 2016
Sub-type Article (original research)
DOI 10.4155/bio.15.260
Open Access Status Not Open Access
Volume 8
Issue 5
Start page 397
End page 411
Total pages 15
Place of publication London, United Kingdom
Publisher Future Science
Language eng
Formatted abstract
Aim: It has been suggested that each member of the family of vitamin D compounds may have different function(s). Therefore, selective quantification of each compound is important in clinical research. Materials & methods: Development and validation attempts of a simultaneous determination method of 12 vitamin D compounds in human blood using precolumn derivatization followed by LC–MS/MS is described. Internal standard calibration with 12 stable isotope labeled analogs was used to correct for matrix effects in MS detector. Results & conclusion: Nine vitamin D compounds were quantifiable in blood samples with detection limits within femtomole levels. Serum (compared with plasma) was found to be a more suitable sample type, and protein precipitation (compared with saponification) a more effective extraction method for vitamin D assay.
Keyword Lc-Ms/ms
Plasma
Protein precipitation
Saponification
Serum
Vitamin D
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Pharmacy Publications
 
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