Culturing murine embryonic organs: pros, cons, tips and tricks

McClelland, Kathryn S. and Bowles, Josephine (2016) Culturing murine embryonic organs: pros, cons, tips and tricks. Differentiation, 91 4-5: 50-56. doi:10.1016/j.diff.2016.01.008


Author McClelland, Kathryn S.
Bowles, Josephine
Title Culturing murine embryonic organs: pros, cons, tips and tricks
Journal name Differentiation   Check publisher's open access policy
ISSN 1432-0436
0301-4681
Publication date 2016-01-16
Year available 2016
Sub-type Article (original research)
DOI 10.1016/j.diff.2016.01.008
Open Access Status Not Open Access
Volume 91
Issue 4-5
Start page 50
End page 56
Total pages 7
Place of publication London, United Kingdom
Publisher Elsevier
Language eng
Subject 1309 Developmental Biology
1307 Cell Biology
1312 Molecular Biology
1306 Cancer Research
Abstract There are three established techniques described for ex vivo culture of the early embryonic organs: filter culture, agar block culture and hanging drop culture. Each of these protocols has advantages and disadvantages; here we assess the merits of each approach. Agar block culture has a long history and has been well described. This method results in good embryonic organ morphology. Filter culture has been used to culture a number of different embryonic organs and there are a variety of filter choices available. The key disadvantage of agar-block and filter based culture is that the large amount of media required can make the approach expensive, especially if biologicals such as growth factors are necessary; in addition, using these methods it can be difficult to track particular samples. Hanging drop culture is most commonly used to enable the aggregation of embryonic stem cells into embryoid bodies but it has also been employed for ex vivo organ culture. This method requires only 40μL of media per drop and isolates every organ to a trackable unit. We describe each of these methods and the use of different medias and provide the user with a matrix to help determine the optimal culture method for their needs. Glass-based culture methods required for live imaging are not discussed here.
Keyword Agar block culture
Culture conditions
Ex vivo
Filter culture
Hanging drop
Organ culture
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID DP140104059
APP1074258
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
Institute for Molecular Bioscience - Publications
 
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