Effects of acetaldehyde on polymerization of microtubule proteins

McKinnon, G, Davidson, M, Dejersey, J, Shanley, B and Ward, L (1987) Effects of acetaldehyde on polymerization of microtubule proteins. Brain Research, 416 1: 90-99. doi:10.1016/0006-8993(87)91500-9

Author McKinnon, G
Davidson, M
Dejersey, J
Shanley, B
Ward, L
Title Effects of acetaldehyde on polymerization of microtubule proteins
Journal name Brain Research   Check publisher's open access policy
ISSN 0006-8993
Publication date 1987-07-21
Year available 1987
Sub-type Article (original research)
DOI 10.1016/0006-8993(87)91500-9
Open Access Status Not yet assessed
Volume 416
Issue 1
Start page 90
End page 99
Total pages 10
Place of publication AMSTERDAM
Language eng
Subject 1309 Developmental Biology
1312 Molecular Biology
2728 Clinical Neurology
2800 Neuroscience
Abstract The in vitro effects of ethanol and acetaldehyde on polymerization of calf brain microtubular proteins (MTP) were examined. While ethanol up to 100 mM had no effect on the polymerization of MTP, acetaldehyde above 0.5 mM had an inhibitory effect. This effect was not dependent on the presence of microtubule-associated proteins (MAPs), since acetaldehyde had a similar effect on the polymerization of highly purified tubulin. Electron microscopy revealed that the number and the length of microtubules at equilibrium was reduced by the presence of acetaldehyde. Acetaldehyde raised the critical concentration for tubulin assembly and caused greater inhibition at lower tubulin concentrations. Acetaldehyde augmented the depolymerizing effects of Ca2+ on preassembled microtubules. In addition, acetaldehyde itself caused depolymerization of microtubules but not only in the absence of MAPs. Long-term (19.5 h) incubation of MTP with acetaldehyde led to significant loss of polymerization ability which could not be reversed by removal of acetaldehyde. This loss of activity was apparently independent of the observed formation of reducible adducts between acetaldehyde and MTP.
Keyword Acetaldehyde
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
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