Direct-write bioprinting of cell-laden methacrylated gelatin hydrogels

Bertassoni, Luiz E., Cardoso, Juliana C., Manoharan, Vijayan, Cristino, Ana L., Bhise, Nupura S., Araujo, Wesleyan A., Zorlutuna, Pinar, Vrana, Nihal E., Ghaemmaghami, Amir M., Dokmeci, Mehmet R. and Khademhosseini, Ali (2014) Direct-write bioprinting of cell-laden methacrylated gelatin hydrogels. Biofabrication, 6 2: 1-11. doi:10.1088/1758-5082/6/2/024105

Author Bertassoni, Luiz E.
Cardoso, Juliana C.
Manoharan, Vijayan
Cristino, Ana L.
Bhise, Nupura S.
Araujo, Wesleyan A.
Zorlutuna, Pinar
Vrana, Nihal E.
Ghaemmaghami, Amir M.
Dokmeci, Mehmet R.
Khademhosseini, Ali
Title Direct-write bioprinting of cell-laden methacrylated gelatin hydrogels
Journal name Biofabrication   Check publisher's open access policy
ISSN 1758-5090
Publication date 2014-04-03
Sub-type Article (original research)
DOI 10.1088/1758-5082/6/2/024105
Open Access Status Not Open Access
Volume 6
Issue 2
Start page 1
End page 11
Total pages 11
Place of publication Bristol, United Kingdom
Publisher Institute of Physics Publishing
Language eng
Abstract Fabrication of three dimensional (3D) organoids with controlled microarchitectures has been shown to enhance tissue functionality. Bioprinting can be used to precisely position cells and cell-laden materials to generate controlled tissue architecture. Therefore, it represents an exciting alternative for organ fabrication. Despite the rapid progress in the field, the development of printing processes that can be used to fabricate macroscale tissue constructs from ECM-derived hydrogels has remained a challenge. Here we report a strategy for bioprinting of photolabile cell-laden methacrylated gelatin (GelMA) hydrogels. We bioprinted cell-laden GelMA at concentrations ranging from 7 to 15% with varying cell densities and found a direct correlation between printability and the hydrogel mechanical properties. Furthermore, encapsulated HepG2 cells preserved cell viability for at least eight days following the bioprinting process. In summary, this work presents a strategy for direct-write bioprinting of a cell-laden photolabile ECM-derived hydrogel, which may find widespread application for tissue engineering, organ printing and the development of 3D drug discovery platforms.
Keyword Bioprinting
Tissue engineering
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
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Citation counts: TR Web of Science Citation Count  Cited 124 times in Thomson Reuters Web of Science Article | Citations
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Created: Fri, 26 Feb 2016, 21:00:10 EST by Anthony Yeates on behalf of Aust Institute for Bioengineering & Nanotechnology