Hydrogel bioprinted microchannel networks for vascularization of tissue engineering constructs

Bertassoni, Luiz E., Cecconi, Martina, Manoharan, Vijayan, Nikkhah, Mehdi, Hjortnaes, Jesper, Cristino, Ana Luiza, Barabaschi, Giada, Demarchi, Danilo, Dokmeci, Mehmet R., Yang, Yunzhi and Khademhosseini, Ali (2014) Hydrogel bioprinted microchannel networks for vascularization of tissue engineering constructs. Lab on a Chip - Miniaturisation for Chemistry and Biology, 14 13: 2202-2211. doi:10.1039/c4lc00030g

Author Bertassoni, Luiz E.
Cecconi, Martina
Manoharan, Vijayan
Nikkhah, Mehdi
Hjortnaes, Jesper
Cristino, Ana Luiza
Barabaschi, Giada
Demarchi, Danilo
Dokmeci, Mehmet R.
Yang, Yunzhi
Khademhosseini, Ali
Title Hydrogel bioprinted microchannel networks for vascularization of tissue engineering constructs
Journal name Lab on a Chip - Miniaturisation for Chemistry and Biology   Check publisher's open access policy
ISSN 1473-0189
Publication date 2014-07-07
Sub-type Article (original research)
DOI 10.1039/c4lc00030g
Open Access Status Not Open Access
Volume 14
Issue 13
Start page 2202
End page 2211
Total pages 10
Place of publication Cambridge, United Kingdom
Publisher Royal Society of Chemistry
Language eng
Abstract Vascularization remains a critical challenge in tissue engineering. The development of vascular networks within densely populated and metabolically functional tissues facilitate transport of nutrients and removal of waste products, thus preserving cellular viability over a long period of time. Despite tremendous progress in fabricating complex tissue constructs in the past few years, approaches for controlled vascularization within hydrogel based engineered tissue constructs have remained limited. Here, we report a three dimensional (3D) micromolding technique utilizing bioprinted agarose template fibers to fabricate microchannel networks with various architectural features within photocrosslinkable hydrogel constructs. Using the proposed approach, we were able to successfully embed functional and perfusable microchannels inside methacrylated gelatin (GelMA), star poly(ethylene glycol-co-lactide) acrylate (SPELA), poly(ethylene glycol) dimethacrylate (PEGDMA) and poly(ethylene glycol) diacrylate (PEGDA) hydrogels at different concentrations. In particular, GelMA hydrogels were used as a model to demonstrate the functionality of the fabricated vascular networks in improving mass transport, cellular viability and differentiation within the cell-laden tissue constructs. In addition, successful formation of endothelial monolayers within the fabricated channels was confirmed. Overall, our proposed strategy represents an effective technique for vascularization of hydrogel constructs with useful applications in tissue engineering and organs on a chip.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Australian Institute for Bioengineering and Nanotechnology Publications
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Citation counts: TR Web of Science Citation Count  Cited 182 times in Thomson Reuters Web of Science Article | Citations
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Created: Fri, 26 Feb 2016, 20:59:26 EST by Anthony Yeates on behalf of Aust Institute for Bioengineering & Nanotechnology