A cost-effective fluorescence mini-microscope for biomedical applications

Zhang, Yu Shrike, Ribas, Joao, Nadhman, Akhtar, Aleman, Julio, Selimovic, Seila, Lesher-Perez, Sasha Cai, Wang, Ting, Manoharan, Vijayan, Shin, Su-Ryon, Damilano, Alessia, Annabi, Nasim, Dokmeci, Mehmet Remzi, Takayama, Shuichi and Khademhosseini, Ali (2015) A cost-effective fluorescence mini-microscope for biomedical applications. Lab on a Chip - Miniaturisation for Chemistry and Biology, 15 18: 3661-3669. doi:10.1039/c5lc00666j

Author Zhang, Yu Shrike
Ribas, Joao
Nadhman, Akhtar
Aleman, Julio
Selimovic, Seila
Lesher-Perez, Sasha Cai
Wang, Ting
Manoharan, Vijayan
Shin, Su-Ryon
Damilano, Alessia
Annabi, Nasim
Dokmeci, Mehmet Remzi
Takayama, Shuichi
Khademhosseini, Ali
Title A cost-effective fluorescence mini-microscope for biomedical applications
Journal name Lab on a Chip - Miniaturisation for Chemistry and Biology   Check publisher's open access policy
ISSN 1473-0189
Publication date 2015-08-03
Sub-type Article (original research)
DOI 10.1039/c5lc00666j
Open Access Status Not Open Access
Volume 15
Issue 18
Start page 3661
End page 3669
Total pages 9
Place of publication Cambridge, United Kingdom
Publisher Royal Society of Chemistry
Language eng
Formatted abstract
We have designed and fabricated a miniature microscope from off-the-shelf components and a webcam, with built-in fluorescence capability for biomedical applications. The mini-microscope was able to detect both biochemical parameters, such as cell/tissue viability (e.g. live/dead assay), and biophysical properties of the microenvironment such as oxygen levels in microfabricated tissues based on an oxygen-sensitive fluorescent dye. This mini-microscope has adjustable magnifications from 8-60×, achieves a resolution as high as <2 μm, and possesses a long working distance of 4.5 mm (at a magnification of 8×). The mini-microscope was able to chronologically monitor cell migration and analyze beating of microfluidic liver and cardiac bioreactors in real time, respectively. The mini-microscope system is cheap, and its modularity allows convenient integration with a wide variety of pre-existing platforms including, but not limited to, cell culture plates, microfluidic devices, and organs-on-a-chip systems. Therefore, we envision its widespread application in cell biology, tissue engineering, biosensing, microfluidics, and organs-on-chips, which can potentially replace conventional bench-top microscopy where long-term in situ and large-scale imaging/analysis is required.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Non HERDC
Australian Institute for Bioengineering and Nanotechnology Publications
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