A study on the encapsulation of an occludin lipophilic derivative in liposomal carriers

Cupri, Sarha, Graziano, Adriana C. E., Cardile, Venera, Skwarczynski, Mariusz, Toth, Istvan and Pignatello, Rosario (2015) A study on the encapsulation of an occludin lipophilic derivative in liposomal carriers. Journal of Liposome Research, 25 4: 287-293. doi:10.3109/08982104.2014.992025

Author Cupri, Sarha
Graziano, Adriana C. E.
Cardile, Venera
Skwarczynski, Mariusz
Toth, Istvan
Pignatello, Rosario
Title A study on the encapsulation of an occludin lipophilic derivative in liposomal carriers
Journal name Journal of Liposome Research   Check publisher's open access policy
ISSN 1532-2394
Publication date 2015-10-02
Year available 2015
Sub-type Article (original research)
DOI 10.3109/08982104.2014.992025
Open Access Status Not Open Access
Volume 25
Issue 4
Start page 287
End page 293
Total pages 7
Place of publication Philadelphia, PA, United States
Publisher Taylor and Francis Ltd
Language eng
Formatted abstract
Many peptides and proteins, although potentially useful for the treatment of various diseases, are hindered in their clinical use by poor oral absorption and rapid enzymatic degradation. One of the available solutions to these problems is to increase the lipophilicity by conjugating the peptides to lipophilic moieties, making them more able to cross the biomembranes by passive transport. Occludin is a 65-kDa integral plasma-membrane protein located at the tight junctions. This protein and the peptide derived from it have potential clinical application for drug delivery. Peptide OP90-103 (1) is a fragment of occludin that shows a very poor oral bioavailability and is highly susceptible to enzymatic degradation. The conjugation of 1 with two lipoamino acid (LAA) moieties has been shown to enhance its lipophilicity and bioavailability, as well as its enzymatic stability. The purpose of this study was to evaluate the possibility of encapsulating fluorescein modified lipidated OP90-103 (2), in unilamellar- (LUV) and multilamellar liposomes (MLV), which have a different composition and surface charge and are produced by different methods. The cell internalization of the carrier systems was evaluated in vitro.
Keyword Cell uptake
Fluorescence microscopy
Lipoamino acids
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Chemistry and Molecular Biosciences
School of Pharmacy Publications
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Created: Tue, 23 Feb 2016, 22:54:16 EST by Anthony Yeates on behalf of Learning and Research Services (UQ Library)