Particle-by-particle charge analysis of DNA-modified nanoparticles using tunable resistive pulse sensing

Blundell, Emma L. C., Vogel, Robert and Platt, Mark (2016) Particle-by-particle charge analysis of DNA-modified nanoparticles using tunable resistive pulse sensing. Langmuir, 32 4: 1082-1090. doi:10.1021/acs.langmuir.5b03024


Author Blundell, Emma L. C.
Vogel, Robert
Platt, Mark
Title Particle-by-particle charge analysis of DNA-modified nanoparticles using tunable resistive pulse sensing
Journal name Langmuir   Check publisher's open access policy
ISSN 1520-5827
0743-7463
Publication date 2016-02-02
Year available 2016
Sub-type Article (original research)
DOI 10.1021/acs.langmuir.5b03024
Open Access Status Not Open Access
Volume 32
Issue 4
Start page 1082
End page 1090
Total pages 9
Place of publication Washington, DC, United States
Publisher American Chemical Society
Language eng
Abstract Resistive pulse sensors, RPS, are allowing the transport mechanism of molecules, proteins and even nanoparticles to be characterized as they traverse pores. Previous work using RPS has shown that the size, concentration and zeta potential of the analyte can be measured. Here we use tunable resistive pulse sensing (TRPS) which utilizes a tunable pore to monitor the translocation times of nanoparticles with DNA modified surfaces. We start by demonstrating that the translocation times of particles can be used to infer the zeta potential of known standards and then apply the method to measure the change in zeta potential of DNA modified particles. By measuring the translocation times of DNA modified nanoparticles as a function of packing density, length, structure, and hybridization time, we observe a clear difference in zeta potential using both mean values and population distributions as a function of the DNA structure. We demonstrate the ability to resolve the signals for ssDNA, dsDNA, small changes in base length for nucleotides between 15 and 40 bases long, and even the discrimination between partial and fully complementary target sequences. Such a method has potential and applications in sensors for the monitoring of nanoparticles in both medical and environmental samples.
Keyword Nanoparticles
Packing density
Particle charge
DNA
Resistive pulse sensing
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Mathematics and Physics
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