Probiotic (Enterococcus faecium) induced responses of the hepatic proteome improves metabolic efficiency of broiler chickens (Gallus gallus)

Zheng, Aijuan, Luo, Jianjie, Meng, Kun, Li, Jianke, Bryden, Wayne L., Chang, Wenhuan, Zhang, Shu, Wang, L. X. N., Liu, Guohua and Yao, Bin (2016) Probiotic (Enterococcus faecium) induced responses of the hepatic proteome improves metabolic efficiency of broiler chickens (Gallus gallus). BMC Genomics, 17 1: 89.1-89.12. doi:10.1186/s12864-016-2371-5


Author Zheng, Aijuan
Luo, Jianjie
Meng, Kun
Li, Jianke
Bryden, Wayne L.
Chang, Wenhuan
Zhang, Shu
Wang, L. X. N.
Liu, Guohua
Yao, Bin
Title Probiotic (Enterococcus faecium) induced responses of the hepatic proteome improves metabolic efficiency of broiler chickens (Gallus gallus)
Formatted title
Probiotic (Enterococcus faecium) induced responses of the hepatic proteome improves metabolic efficiency of broiler chickens (Gallus gallus)
Journal name BMC Genomics   Check publisher's open access policy
ISSN 1471-2164
Publication date 2016-02-01
Year available 2016
Sub-type Article (original research)
DOI 10.1186/s12864-016-2371-5
Open Access Status DOI
Volume 17
Issue 1
Start page 89.1
End page 89.12
Total pages 12
Place of publication London, United Kingdom
Publisher BioMed Central
Language eng
Formatted abstract
Background: The liver plays important roles in nutrient metabolism, detoxification and immunity. Enterococcus faecium (E. faecium) is a probiotic that has been shown to have positive effects on broiler production. However, its molecular effects on liver metabolism have not been characterized. This study aims to further identify the biological roles of E. faecium by characterizing the hepatic proteomic changes of broilers (Gallus gallus) fed E. faecium using two-dimensional fluorescence difference gel electrophoresis (2-D DIGE) and mass spectrometry (MS).

Results: Thirty-three proteins (50 protein spots) involved in nutrient metabolism, immunity and the antioxidant system were shown to be differentially expressed in the liver of broilers fed E. faecium than from birds not fed the probiotic. The biological processes of sulphur amino acids, vitamin and cellular hormone metabolism, sulphur compound biosynthesis and protein tetramerization were enhanced in the liver of broilers fed E. faecium. However, proteins involved in calcium ion flux, cell redox homeostasis and platelet activation related to hepatic immune responses were down-regulated in broilers fed E. faecium. These results indicate that the supplementation of poultry feed with E. faecium may alter the partitioning of nutrients and promote optimal nutrient utilization.

Conclusions
: This study assists in unraveling the molecular effects of the dietary probiotic, E. faecium, in the liver of broiler chickens. It shows that the probiotic improves the metabolism of nutrients and decreases inflammatory responses. Our findings extend previous knowledge of the mechanism of dietary probiotic action and provide new findings for research and future probiotic development.
Keyword Broiler chicken (Gallus gallus)
Enterococcus faecium
Liver
Proteome
Probiotics
Gene expression
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Agriculture and Food Sciences
 
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