Assessment of a positive selection technique using an avidin column to isolate human peripheral blood T cell subsets

Davis, LS, McIlraith, MJ, Paecheo, T, Becker, B, Adix, LM, Thomas, R, Wacholtz, MC and Lipsky, PE (1994) Assessment of a positive selection technique using an avidin column to isolate human peripheral blood T cell subsets. Journal of Immunological Methods, 175 2: 247-257. doi:10.1016/0022-1759(94)90367-0


Author Davis, LS
McIlraith, MJ
Paecheo, T
Becker, B
Adix, LM
Thomas, R
Wacholtz, MC
Lipsky, PE
Title Assessment of a positive selection technique using an avidin column to isolate human peripheral blood T cell subsets
Journal name Journal of Immunological Methods   Check publisher's open access policy
ISSN 0022-1759
Publication date 1994-10-14
Year available 1994
Sub-type Article (original research)
DOI 10.1016/0022-1759(94)90367-0
Volume 175
Issue 2
Start page 247
End page 257
Total pages 11
Publisher ELSEVIER SCIENCE BV
Language eng
Subject 1305 Biotechnology
2403 Immunology
Abstract The current studies were designed to assess a new technique for positively selecting human T cells from whole peripheral blood mononuclear cells using the minimal amount of monoclonal antibody required to bind the T cell to an avidin column indirectly via a biotin-conjugated secondary antibody. Positive selection of T cells has previously been avoided because the saturating amounts of antibodies required for other isolation procedures can lead to aberrant results in assay of T cell activation and function. The avidin column technique for obtaining purified T cell subsets was compared to a multi-step procedure that included negative selection panning. The positive selection technique was easily performed within 4 h whereas the negative selection technique required a minimum of 12 h to complete. The avidin column technique proved to be a rapid and simple method for isolating T cell subsets of high purity and normal functional capabilities. Since minimal amounts of monoclonal antibodies were used for the purification protocol, no consistent inhibitory or stimulatory effect of the residual antibody was noted in assays of activation and proliferation of positively selected T cells compared to T cells isolated by negative selection panning.
Keyword (Human)
Activation and function
Avidin column
Positive and negative selection
T lymphocyte
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
 
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