Siglec-h on activated microglia for recognition and engulfment of glioma cells

Kopatz, Jens, Beutner, Clara, Welle, Kristian, Bodea, Liviu G., Reinhardt, Julia, Claude, Janine, Linnartz-Gerlach, Bettina and Neumann, Harald (2013) Siglec-h on activated microglia for recognition and engulfment of glioma cells. GLIA, 61 7: 1122-1133. doi:10.1002/glia.22501

Author Kopatz, Jens
Beutner, Clara
Welle, Kristian
Bodea, Liviu G.
Reinhardt, Julia
Claude, Janine
Linnartz-Gerlach, Bettina
Neumann, Harald
Title Siglec-h on activated microglia for recognition and engulfment of glioma cells
Journal name GLIA   Check publisher's open access policy
ISSN 0894-1491
Publication date 2013-07-01
Year available 2013
Sub-type Article (original research)
DOI 10.1002/glia.22501
Open Access Status Not yet assessed
Volume 61
Issue 7
Start page 1122
End page 1133
Total pages 12
Place of publication Hoboken, NJ, United States
Publisher John Wiley & Sons
Language eng
Abstract Sialic-acid-binding immunoglobulin-like lectin-h (Siglec-h) is a recently identified mouse-specific CD33-related Siglec that signals via DAP12/TYROBP. Expression of Siglec-h has been observed on plasmacytoid dendritic cells and microglia, but the ligand and the function of Siglec-h remained elusive. Here, we demonstrate gene transcription and protein expression of Siglec-h by mouse microglia after interferon-γ treatment or polarization into a M1-subtype. Microglial Siglec-h acted as phagocytosis receptor since targeting of microsphere beads to Siglec-h triggered their uptake into the microglia. The extracellular domain of Siglec-h protein bound to mouse glioma lines, but not to astrocytes or other normal mouse cells. Microglial cells stimulated to express Siglec-h engulfed intact glioma cells without prior induction of apoptosis and slightly reduced glioma cell number in culture. Phagocytosis of glioma cells by activated microglia was dependent on Siglec-h and its adapter molecule DAP12. Thus, data show that M1-polarized microglial cells can engulf glioma cells via a DAP12-mediated Siglec-h dependent mechanism.
Keyword Glioma
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: Centre for Ageing Dementia Research Publications
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