Acute restraint stress induces rapid changes in central redox status and protective antioxidant genes in rats

Spiers, Jereme G., Chen, Hsiao-Jou Cortina, Cuffe, James S. M., Sernia, Corrado and Lavidis, Nickolas A. (2016) Acute restraint stress induces rapid changes in central redox status and protective antioxidant genes in rats. Psychoneuroendocrinology, 67 104-112. doi:10.1016/j.psyneuen.2016.02.005

Author Spiers, Jereme G.
Chen, Hsiao-Jou Cortina
Cuffe, James S. M.
Sernia, Corrado
Lavidis, Nickolas A.
Title Acute restraint stress induces rapid changes in central redox status and protective antioxidant genes in rats
Journal name Psychoneuroendocrinology   Check publisher's open access policy
ISSN 0306-4530
Publication date 2016-05-01
Sub-type Article (original research)
DOI 10.1016/j.psyneuen.2016.02.005
Open Access Status Not Open Access
Volume 67
Start page 104
End page 112
Total pages 9
Place of publication Kidlington, Oxford, United Kingdom
Publisher Pergamon Press
Language eng
Subject 2712 Endocrinology, Diabetes and Metabolism
1310 Endocrinology
2807 Endocrine and Autonomic Systems
2738 Psychiatry and Mental health
2803 Biological Psychiatry
Abstract The stress-induced imbalance in reduction/oxidation (redox) state has been proposed to play a major role in the etiology of neurological disorders. However, the relationship between psychological stress, central redox state, and potential protective mechanisms within specific neural regions has not been well characterized. In this study, we have used an acute psychological stress to demonstrate the dynamic changes that occur in the redox system of hippocampal and striatal tissue. Outbred male Wistar rats were subject to 0 (control), 60, 120, or 240 min of acute restraint stress and the hippocampus and striatum were cryodissected for redox assays and relative gene expression. Restraint stress significantly elevated oxidative status and lipid peroxidation, while decreasing glutathione ratios overall indicative of oxidative stress in both neural regions. These biochemical changes were prevented by prior administration of the glucocorticoid receptor antagonist, RU-486. The hippocampus also demonstrated increased glutathione peroxidase 1 and 4 antioxidant expression which was not observed in the striatum, while both regions displayed robust upregulation of the antioxidant, metallothionein 1a. This was observed with concurrent upregulation of 11-hydroxysteroid dehydrogenase 1, a local reactivator of corticosterone, in addition to decreased expression of the cytosolic regulatory subunit of superoxide-producing enzyme, NADPH-oxidase. Together, this study demonstrates distinctive regional redox profiles following acute stress exposure, in addition to identifying differential capabilities in managing oxidative challenges via altered antioxidant gene expression in the hippocampus and striatum.
Keyword Acute stress
Glucocorticoid receptor
Glutathione peroxidase
Oxidative stress
Redox status
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
School of Biomedical Sciences Publications
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Citation counts: TR Web of Science Citation Count  Cited 6 times in Thomson Reuters Web of Science Article | Citations
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Created: Wed, 17 Feb 2016, 01:04:07 EST by Cortina Chen on behalf of School of Biomedical Sciences