Cloning and sequence analysis of RNA-2 of a mechanically transmitted UK isolate of barley mild mosaic bymovirus (BaMMV)

Jacobi, V, Peerenboom, E, Schenk, PM, Antoniw, JF, Steinbiss, HH and Adams, MJ (1995) Cloning and sequence analysis of RNA-2 of a mechanically transmitted UK isolate of barley mild mosaic bymovirus (BaMMV). Virus Research, 37 2: 99-111. doi:10.1016/0168-1702(95)00024-K


Author Jacobi, V
Peerenboom, E
Schenk, PM
Antoniw, JF
Steinbiss, HH
Adams, MJ
Title Cloning and sequence analysis of RNA-2 of a mechanically transmitted UK isolate of barley mild mosaic bymovirus (BaMMV)
Journal name Virus Research   Check publisher's open access policy
ISSN 0168-1702
Publication date 1995-01-01
Year available 1995
Sub-type Article (original research)
DOI 10.1016/0168-1702(95)00024-K
Open Access Status
Volume 37
Issue 2
Start page 99
End page 111
Total pages 13
Place of publication AMSTERDAM
Publisher ELSEVIER SCIENCE BV
Language eng
Subject 1306 Cancer Research
2406 Virology
2725 Infectious Diseases
1312 Molecular Biology
Abstract A mutant of the 'Streatley' isolate of barley mild mosaic bymovirus was selected from the original field isolate by repeated mechanical incoulation. Unlike the wild-type barley mild mosaic virus, which is transmitted by the soilborne fungus Polymyxa graminis, the mutant could not be transmitted by this vector. RNA-2 of the mutant virus was shorter than that of the wild-type virus suggesting that a deletion of part of the genome segment had occurred. The nucleotide sequence of the mutant RNA-2 was determined and revealed a high degree of homology with the RNA-2 of a German BaMMV field isolate. The deletion comprises 1092 nucleotides and is located in the 3′-terminal part of the coding region. The 34-kDa truncated form of the C-terminal protein is less than half the size of the corresponding protein of full-length BaMMV RNA-2. Taken together, the sequence data and results of biological experiments suggest an essential role of the C-terminal protein for fungus transmission of BaMMV.
Keyword Barley mild mosaic virus (BaMMV)
Bymoviruses
Fungus transmission
Nucleotide sequence
RNA-2 deletion
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
 
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