Association of two barley yellow mosaic virus (RNA 2) encoded proteins with cytoplasmic inclusion bodies revealed by immunogold localisation

Schenk, PM, Steinbiss, HH, Muller, B and Schmitz, K (1993) Association of two barley yellow mosaic virus (RNA 2) encoded proteins with cytoplasmic inclusion bodies revealed by immunogold localisation. Protoplasma, 173 3-4: 113-122. doi:10.1007/BF01379000


Author Schenk, PM
Steinbiss, HH
Muller, B
Schmitz, K
Title Association of two barley yellow mosaic virus (RNA 2) encoded proteins with cytoplasmic inclusion bodies revealed by immunogold localisation
Journal name Protoplasma   Check publisher's open access policy
ISSN 0033-183X
Publication date 1993-01-01
Sub-type Article (original research)
DOI 10.1007/BF01379000
Volume 173
Issue 3-4
Start page 113
End page 122
Total pages 10
Publisher Springer-Verlag
Language eng
Subject 1307 Cell Biology
1110 Nursing
Abstract Antisera were raised against the RNA 2-encoded proteins of 28 kDa and 70 kDa of barley yellow mosaic virus (BaYMV) by using the corresponding cDNA sequences of a German isolate for protein overexpression in Escherichia coli BL 21 and subsequent purification. The proposed processing of a 98 kDa precursor polyprotein encoded by the long open reading frame of RNA 2 to two proteins of 28 kDa and 70 kDa could be confirmed by immunoprecipitation of the in vitro transcribed and translated cDNA-clone of RNA 2 and Western blot analysis of fragmentated protein extracts of BaYMV-infected winter barley plants. In situ localisation studies of infected leaf tissue using immunogold labeling techniques for electron microscopy revealed that both viral proteins of BaYMV (RNA 2) were associated with the crystal-like cytoplasmic inclusion bodies. No other parts of the cells and no other inclusions (pinwheelstructures or aggregated virus particles) showed any gold labeling when the 28 kDa and 70 kDa antisera were used. We suppose that both RNA 2-encoded proteins take part in the formation of the crystal-like cytoplasmic inclusion bodies which are the most dominant structures in the cytoplasm of BaYMV-infected tissue. Possible functions of the 28 kDa and 70 kDa protein of BaYMV (RNA 2) are discussed.
Keyword Barley yellow mosaic virus
Cytoplasmic inclusion bodies
Electron microscopy
Hordeum vulgare
Immunogold labeling
RNA 2-encoded proteins
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
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