Immunopositive GABAergic neural sites display nitric oxide synthase-related NADPH diaphorase activity in the human colon

Nichols K., Staines W., Wu J.-Y. and Krantis A. (1995) Immunopositive GABAergic neural sites display nitric oxide synthase-related NADPH diaphorase activity in the human colon. Journal of the Autonomic Nervous System, 50 3: 253-262. doi:10.1016/0165-1838(94)00096-3


Author Nichols K.
Staines W.
Wu J.-Y.
Krantis A.
Title Immunopositive GABAergic neural sites display nitric oxide synthase-related NADPH diaphorase activity in the human colon
Journal name Journal of the Autonomic Nervous System   Check publisher's open access policy
ISSN 0165-1838
Publication date 1995-01-03
Sub-type Article (original research)
DOI 10.1016/0165-1838(94)00096-3
Volume 50
Issue 3
Start page 253
End page 262
Total pages 10
Subject 1314 Physiology
2728 Clinical Neurology
2800 Neuroscience
Abstract In the enteric nervous system, γ-aminobutyric acid (GABA) is a transmitter of interneurons which are proposed to innervate excitatory and inhibitory motor neurons. Nitric oxide (NO) is a putative transmitter of enteric inhibitory motor nerves targeted by GABA. In addition, NO is synthesized by a variety of enteric nerves throughout the gut wall indicative of its potential to be a transmitter of other nerve types, including interneurons. We sought to determine if some populations of nitrergic neurons are interneurons in human infant colon. As enteric neural GABA is exclusive to interneurons, colocalization with NO synthase-related NADPH diaphorase was examined. GABA-transaminase (GABA-T) immunohistochemistry was used to identify GABAergic neurons and a histochemical protocol was used as a marker of neuronal NO synthase-related NADPH diaphorase activity in enteric layers. GABA-T immunoreactive neurons were seen in the ganglionated nerve networks of the myenteric and submucosal layers. GABA-T immunoreactive fibres were also present in the longitudinal and circular muscle layers. A subpopulation of GABA-T immunoreactive neurons within both the myenteric and submucosal ganglia express NO synthase-related activity. This colocalization extends further to a subpopulation of fibers within the muscle layers. These findings strongly suggest that in addition to its role in inhibitory motor neurons, NO may also be a transmitter of enteric interneurons.
Keyword (Human)
Colocalization
Colon
Enteric
GABA transaminase
Immunohistochemistry
NADPH-diaphorase
Nitric oxide
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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Created: Wed, 10 Feb 2016, 17:51:53 EST by Dr Kim Nichols