Genetic Characterization of the Chlorothricin Gene Cluster as a Model for Spirotetronate Antibiotic Biosynthesis

Jia, XY, Tian, ZH, Shao, L, Qu, XD, Zhao, QF, Tang, J, Tang, GL and Liu, W (2006) Genetic Characterization of the Chlorothricin Gene Cluster as a Model for Spirotetronate Antibiotic Biosynthesis. Chemistry and Biology, 13 6: 575-585. doi:10.1016/j.chembiol.2006.03.008


Author Jia, XY
Tian, ZH
Shao, L
Qu, XD
Zhao, QF
Tang, J
Tang, GL
Liu, W
Title Genetic Characterization of the Chlorothricin Gene Cluster as a Model for Spirotetronate Antibiotic Biosynthesis
Journal name Chemistry and Biology   Check publisher's open access policy
ISSN 1074-5521
Publication date 2006-01-01
Sub-type Article (original research)
DOI 10.1016/j.chembiol.2006.03.008
Volume 13
Issue 6
Start page 575
End page 585
Total pages 11
Language eng
Subject 1605 Policy and Administration
Abstract The biosynthetic gene cluster for chlorothricin (CHL) was localized to a 122 kb contiguous DNA from Streptomyces antibioticus DSM 40725, and its involvement in CHL biosynthesis was confirmed by gene inactivation and complementation. Bioinformatic analysis of the sequenced 111.989 kb DNA region revealed 42 open reading frames, 35 of which were defined to constitute the CHL gene cluster. An assembly model for CHL biosynthesis from D-olivose, 2-methoxy-5-chloro-6-methylsalicyclic acid, and chlorothricolide building blocks was proposed. This work represents cloning of a gene cluster for spirotetronate antibiotic biosynthesis and sets the stage to investigate the unusual macrolide biosynthesis including tandem Diels-Alder cyclizations, Baeyer-Villiger oxidation, and incorporation of an enoylpyruvate unit.
Keyword CHEMBIO
MICROBIO
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
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Citation counts: TR Web of Science Citation Count  Cited 96 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 96 times in Scopus Article | Citations
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