Angiotensinogen is secreted by pure rat neuronal cell cultures

Thomas, WG, Greenland, KJ, Shinkel, TA and Sernia, C (1992) Angiotensinogen is secreted by pure rat neuronal cell cultures. Brain Research, 588 2: 191-200. doi:10.1016/0006-8993(92)91575-Y


Author Thomas, WG
Greenland, KJ
Shinkel, TA
Sernia, C
Title Angiotensinogen is secreted by pure rat neuronal cell cultures
Journal name Brain Research   Check publisher's open access policy
ISSN 0006-8993
Publication date 1992-08-21
Sub-type Article (original research)
DOI 10.1016/0006-8993(92)91575-Y
Volume 588
Issue 2
Start page 191
End page 200
Total pages 10
Language eng
Subject 1309 Developmental Biology
1312 Molecular Biology
2728 Clinical Neurology
2800 Neuroscience
Abstract Previous studies are divided between those which support a neuroglial (astrocyte) source for brain angiotensinogen and those which indicate that both astrocytes and neurones synthesize the precursor of angiotensin II. In this study, separate cultures of astrocytes and neuronal cells were prepared and established as being essentially pure by appropriate immunocytochemical cell markers. Angiotensinogen production by these cultures, as measured by a direct radioimmunoassay, was 20.74 ± 3.62 ng angiotensinogen/106 cells/24 h (mean ± S.D., n = 8) for astrocytes and 4.39 ± 0.94 ng/106 cells/24 h (mean ± S.D., n = 29) for neurones. Angiotensinogen secretion from both cell types was unaffected by treatments which stimulate the regulatory secretory pathway by modulating intracellular cAMP levels. In contrast, it was reduced from 23.20 ± 2.14 to 8.14 ± 1.31 ng/106 cells/24 h (S.E.M., n = 7) in astrocyte cultures by the constitutive pathway inhibitor, monensin. Angiotensinogen secreted by astrocytes and neurones was compared to pure angiotensinogen and that in plasma and cerebrospinal fluid (CSF) by cation-exchange mono S column chromatography. Pure angiotensinogen eluted as two separate peaks corresponding to the major forms of plasma angiotensinogen, whereas angiotensinogen in CSF and culture media coeluted with a third minor form of plasma angiotensinogen. It was concluded that neuronal cells as well as astrocytes secrete angiotensinogen which is distinctly different from plasma angiotensinogen.
Keyword Angiotensinogen
Astrocyte
Cation exchange chromatography
Cell culture
Immunocytochemistry
Neuron
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
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