Cloning and characterisation of a glutamate dehydrogenase cDNA from tomato (Lycopersicon esculentum L.)

Purnell, MP, Stewart, GR and Botella, JR (1997) Cloning and characterisation of a glutamate dehydrogenase cDNA from tomato (Lycopersicon esculentum L.). Gene, 186 2: 249-254. doi:10.1016/S0378-1119(96)00716-0


Author Purnell, MP
Stewart, GR
Botella, JR
Title Cloning and characterisation of a glutamate dehydrogenase cDNA from tomato (Lycopersicon esculentum L.)
Journal name Gene   Check publisher's open access policy
ISSN 0378-1119
Publication date 1997-02-28
Year available 1997
Sub-type Article (original research)
DOI 10.1016/S0378-1119(96)00716-0
Open Access Status Not yet assessed
Volume 186
Issue 2
Start page 249
End page 254
Total pages 6
Place of publication AMSTERDAM
Publisher Elsevier BV
Language eng
Subject 1311 Genetics
Abstract A full-length cDNA (legdh1)has been cloned encoding glutamate dehydrogenase (GDH) from tomato (Lycopersicon esculentum L.). legdh1 is 1568 bp long and contains an open reading frame encoding a 44.8 kDa polypeptide with a putative mitochondrial-matrix-targeting pre-sequence at its N-terminus. Southern analysis indicates the existence of one copy of legdh1 per haploid genome, and no closely related genes were detected by Southern analysis at low stringency. We hypothesise that in tomato, the two GDH subunits may arise from post-transcriptional modifications of a single gene. Northern analysis reveals high expression of legdh1 in roots, lower levels of expression in stems, flowers and leaves, and no detectable expression in fruits. In general, there was no correlation between steady-state mRNA level and protein activity in the tissues analysed, again suggesting the importance of post-transcriptional events in the regulation of GDH. Comparison of cloned plant GDH proteins reveals a high degree of homology throughout the sequence except for a very specific, highly divergent region.
Keyword GDH
Glutamate synthase
Glutamine synthetase
Mitochondria
Nitrogen
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Scopus Import - Archived
 
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