Identification and characterisation of two 1-aminocyclopropane-1-carboxylate (ACC) synthase cDNAs expressed during papaya (Carica papaya) fruit ripening

Mason, MG and Botella, JR (1997) Identification and characterisation of two 1-aminocyclopropane-1-carboxylate (ACC) synthase cDNAs expressed during papaya (Carica papaya) fruit ripening. Australian Journal of Plant Physiology, 24 2: 239-244. doi:10.1071/PP96111


Author Mason, MG
Botella, JR
Title Identification and characterisation of two 1-aminocyclopropane-1-carboxylate (ACC) synthase cDNAs expressed during papaya (Carica papaya) fruit ripening
Journal name Australian Journal of Plant Physiology   Check publisher's open access policy
ISSN 0310-7841
Publication date 1997-01-01
Year available 1997
Sub-type Article (original research)
DOI 10.1071/PP96111
Open Access Status Not yet assessed
Volume 24
Issue 2
Start page 239
End page 244
Total pages 6
Place of publication COLLINGWOOD
Publisher C S I R O PUBLICATIONS
Language eng
Abstract The cloning and characterisation of two cDNAs (capacs1 and capacs2) encoding ACC synthase in papaya (Carica papaya L.) is described. capacs1 is 1104 bp long encoding 368 amino acids. capacs2 is 1098 bp long encoding 366 amino acids. The proteins encoded by both cDNAs contain the highly conserved active site of ACC synthases as well as 10 (capacs1) or 11 (capacs2) of the 12 amino acid residues conserved in most aminotransferases. Southern analyses indicate that capacs1 and capacs2 are present in the papaya genome as single copy genes. Both genes show very different expression patterns during fruit ripening. capacs1 mRNA levels are high in mature green fruits, decreasing steadily during ripening, whereas capacs2 mRNA levels are undetectable in mature green fruits but experience a dramatic increase with the onset of ripening and remain high throughout the various stages of ripening.
Keyword 1-aminocyclopropane-1-carboxylate (ACC) synthase
ethylene
fruit ripening
Polymerase Chain Reaction (PCR)
Carica papaya
Ethylene Biosynthesis
Rna
Tomato
Gene
Senescence
Cloning
Plants
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
 
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