Cloning and characterisation of ripening-induced ethylene biosynthetic genes from non-climacteric pineapple (Ananas comosus) fruits

Cazzonelli, CI, Cavallaro, AS and Botella, JR (1998) Cloning and characterisation of ripening-induced ethylene biosynthetic genes from non-climacteric pineapple (Ananas comosus) fruits. Australian Journal of Plant Physiology, 25 5: 513-518. doi:10.1071/PP98013


Author Cazzonelli, CI
Cavallaro, AS
Botella, JR
Title Cloning and characterisation of ripening-induced ethylene biosynthetic genes from non-climacteric pineapple (Ananas comosus) fruits
Journal name Australian Journal of Plant Physiology   Check publisher's open access policy
ISSN 0310-7841
Publication date 1998-01-01
Year available 1998
Sub-type Article (original research)
DOI 10.1071/PP98013
Open Access Status Not yet assessed
Volume 25
Issue 5
Start page 513
End page 518
Total pages 6
Place of publication COLLINGWOOD
Publisher C S I R O PUBLICATIONS
Language eng
Subject 1100 Agricultural and Biological Sciences
Abstract To gain a better understanding of non-climacteric fruit ripening, pineapple was used as a model system to clone and characterise two ripening-inducible cDNAs coding for two enzymes of the ethylene biosynthetic pathway, 1-aminocyclopropane-1-carboxylate (ACC) synthase (acacc-1) and 1-aminocyclopropane-1-carboxylate oxidase (acaco-1) respectively. Due to the extreme acidity and high polyphenolic content of pineapple fruits, a method was optimised for the extraction of high quality RNA from fruit tissue. acacc-1 is a 1080 bp ACC synthase cDNA fragment encoding 360 amino acids including 10 of the 12 amino acid residues conserved in all aminotransferases. Comparison of the deduced amino acid sequence with previously reported ACC synthases shows between 52 and 67% similarity at the protein level. Southern analysis suggests the presence of only one copy of acacc-1 in the pineapple genome. Although some acacc-1 expression is detected in green fruits, there is a 16-fold increase in the level of acacc-1 in ripe fruit tissue, acaco-1 is a partial length cDNA clone of 611 bp which codes for 203 amino acids representing approximately 66% of the ACC oxidase open reading frame. Southern analysis suggests the presence of one or two copies of the gene in the pineapple genome. Northern analysis shows the expression of acaco-1 to be highly induced in wounded leaf tissue and to a lesser extent in ripening fruit tissue. The accumulation of ACC-synthase and ACC oxidase mRNAs during pineapple fruit ripening raises new questions about the putative role of ethylene during non-climacteric fruit ripening.
Keyword 1-aminocyclopropane-1-carboxylate (ACC) synthase
ACC oxidase
ethylene
non-climacteric
fruit ripening
polymerase chain reaction (PCR)
Ananas comosus
Encoding 1-Aminocyclopropane-1-Carboxylate Synthase
2 Genes
Rna
Tomato
Expression
Plants
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
 
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