Calcium-dependent protein kinase gene expression in response to physical and chemical stimuli in mungbean (Vigna radiata)

Botella, JR, Arteca, JM, Somodevilla, M and Arteca, RN (1996) Calcium-dependent protein kinase gene expression in response to physical and chemical stimuli in mungbean (Vigna radiata). Plant Molecular Biology, 30 6: 1129-1137. doi:10.1007/BF00019547


Author Botella, JR
Arteca, JM
Somodevilla, M
Arteca, RN
Title Calcium-dependent protein kinase gene expression in response to physical and chemical stimuli in mungbean (Vigna radiata)
Journal name Plant Molecular Biology   Check publisher's open access policy
ISSN 0167-4412
Publication date 1996-03-01
Year available 1996
Sub-type Article (original research)
DOI 10.1007/BF00019547
Open Access Status Not yet assessed
Volume 30
Issue 6
Start page 1129
End page 1137
Total pages 9
Place of publication DORDRECHT
Publisher KLUWER ACADEMIC PUBL
Language eng
Abstract Protein kinases are important in eukaryotic signal transduction pathways. In this study we designed degenerate oligonucleotides corresponding to two conserved regions of protein kinases and using the polymerase chain reaction (PCR) have amplified a 141 bp fragment of DNA from mungbeans (Vigna radiata Rwilcz cv. Berken). Sequence analysis of the PCR products indicates that they encode several putative protein kinases with respect to their identity with other known plant protein kinases. Using one of the six fragments (CPK3-8), we isolated a 2022 bp cDNA (VrCDPK-1) from a Vigna radiata lambda gt11 library. Vr CDPK-1 has a 96 bp 5'-untranslated region and a 465 bp 3'-untranslated region and an open reading frame of 1461 bp. VrCDPK-1 contains all of the conserved regions commonly found in calcium dependent protein kinases (CDPK). VrCDPK-1 shares 24 to 89% sequence identity with previously reported sequences for plant CDPKs at the protein level. Southern analysis revealed the presence of several copies of the CDPK gene. VrCDPK-1 expression was stimulated when mungbean cuttings were treated with CaCl2, while treatment with MgCl2 had no effect. We are reporting for the first time a CDPK gene in mungbean which is inducible by mechanical strain. Cuttings treated with indole-3-acetic acid (IAA) or subjected to salt stress showed an increase in VrCDPK-1 expression. There was a dramatic stimulation in VrCDPK-1 expression 6 h after cuttings were treated with cycloheximide.
Keyword calcium
cycloheximide
indole-3-acetic acid (IAA)
mechanical strain
protein kinase
salt stress
touch
Vigna radiata
Arabidopsis-Thaliana
Messenger-Rnas
Calmodulin
Cdna
Inhibitors
Plants
Auxin
Touch
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
 
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