Identification and characterization of three putative genes for 1-aminocyclopropane-1-carboxylate synthase from etiolated mung bean hypocotyl segments

Botella, JR, Schlagnhaufer, CD, Arteca, RN and Phillips, AT (1992) Identification and characterization of three putative genes for 1-aminocyclopropane-1-carboxylate synthase from etiolated mung bean hypocotyl segments. Plant Molecular Biology, 18 4: 793-797. doi:10.1007/BF00020022


Author Botella, JR
Schlagnhaufer, CD
Arteca, RN
Phillips, AT
Title Identification and characterization of three putative genes for 1-aminocyclopropane-1-carboxylate synthase from etiolated mung bean hypocotyl segments
Journal name Plant Molecular Biology   Check publisher's open access policy
ISSN 0167-4412
Publication date 1992-01-01
Sub-type Article (original research)
DOI 10.1007/BF00020022
Volume 18
Issue 4
Start page 793
End page 797
Total pages 5
Publisher Kluwer Academic Publishers
Language eng
Subject 1303 Specialist Studies in Education
1300 Biochemistry, Genetics and Molecular Biology
Abstract The polymerase chain reaction (PCR) was used to produce 3 putative clones for ACC synthase from etiolated mung bean (Vigna radiata Rwilcz cv. Berken) hypocotyls. This was accomplished by utilizing genomic DNA from mung bean and degenerate primers made from information derived from highly conserved regions of ACC synthase from different plant tissues. The total length of pMAC-1, pMAC-2 and pMAC-3 are 308, 321, and 326 bp, respectively, all of which code for 68 amino acids. The introns for pMAC-1, pMAC-2 and pMAC-3 are 92, 105, and 110 bp, respectively. The degrees of homology at the DNA level for each of these clones is ca. 80% in their coding region and ca. 50% in their respective introns. This is the first report providing evidence that there are at least 3 genes for ACC synthase in etiolated mung bean.
Keyword 1-aminocyclopropane-1-carboxylic acid (ACC)
ACC synthase
ethylene
polymerase chain reaction (PCR)
S-adenosylmethionine (AdoMet)
Vigna radiata
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
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