Proteomic analysis of normal and malignant prostate tissue to identify novel proteins lost in cancer

Meehan, K. L., Holland, J. W. and Dawkins, H. J. S. (2002) Proteomic analysis of normal and malignant prostate tissue to identify novel proteins lost in cancer. Prostate, 50 1: 54-63. doi:10.1002/pros.10032


Author Meehan, K. L.
Holland, J. W.
Dawkins, H. J. S.
Title Proteomic analysis of normal and malignant prostate tissue to identify novel proteins lost in cancer
Journal name Prostate   Check publisher's open access policy
ISSN 0270-4137
Publication date 2002-01-01
Sub-type Article (original research)
DOI 10.1002/pros.10032
Open Access Status Not yet assessed
Volume 50
Issue 1
Start page 54
End page 63
Total pages 10
Place of publication New York
Publisher Wiley-liss
Language eng
Abstract BACKGROUND. Alterations of important protein pathways, including loss of prostate secretory granules, and disruption of the prostatic secretory pathway have been identified as early events in malignancy. In this study, proteomics was used to map the differences in protein expression between normal and malignant prostate tissues and to identify and analyze differentially expressed proteins in human prostate tissue with particular regard to the proteins lost in malignancy. METHODS. Small quantities of normal and malignant prostate tissue were taken fresh from 34 radical prostatectomy cases. After histological examination, proteins were solubilized from selected tissues and separated using two-dimensional electrophoresis. Using image analysis, the proteome of normal and malignant tissues were mapped and differentially expressed proteins (present in normal and absent in malignant tissue) were identified and subsequently analyzed using peptide mass finger printing and N-terminal sequencing. Western blotting and immunohistochemistry were performed to examine expression profiles and tissue localization of candidate proteins. RESULTS. Comparison of protein maps of normal and malignant prostate were used to identify 20 proteins which were lost in malignant transformation, including prostate specific antigen (PSA), alpha-l antichymotrypsin (ACT), haptoglobin, and lactoylglutathione lyase. Three of the 20 had not previously been reported in human prostate tissue (Ubiquitin-like NEDD8, calponin, and a follistatin-related protein). Western blotting confirmed differences in the expression profiles of NEDD8 and calponin, and immunohistochemistry demonstrated differences in the cellular localization of these two proteins in normal and malignant prostate glands. CONCLUSIONS. The expression of NEDD8, calponin, and the follistatin-related protein in normal prostate tissues is a novel finding and the role of these important functional proteins in normal prostate and their loss or reduced expression in prostate malignancy warrants further investigations. (C) 2002 Wiley-Liss, Inc.
Keyword Endocrinology & Metabolism
Urology & Nephrology
Nedd8
Calponin
Prostate Secretory Granules
Prostate Cancer
Carcinoma
Expression
Adenocarcinoma
Identification
Tropomyosin
Specificity
Mortality
Calponin
Antigen
Cloning
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collections: Excellence in Research Australia (ERA) - Collection
Institute for Molecular Bioscience - Publications
 
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Created: Mon, 13 Aug 2007, 22:44:45 EST