Stable non-covalent labeling of layered silicate nanoparticles for biological imaging

Mortimer, Gysell M., Jack, Kevin S., Musumeci, Anthony W., Martin, Darren J. and Minchin, Rodney F. (2016) Stable non-covalent labeling of layered silicate nanoparticles for biological imaging. Materials Science and Engineering C, 61 674-680. doi:10.1016/j.msec.2015.12.047

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Author Mortimer, Gysell M.
Jack, Kevin S.
Musumeci, Anthony W.
Martin, Darren J.
Minchin, Rodney F.
Title Stable non-covalent labeling of layered silicate nanoparticles for biological imaging
Journal name Materials Science and Engineering C   Check publisher's open access policy
ISSN 0928-4931
Publication date 2016-04-01
Year available 2015
Sub-type Article (original research)
DOI 10.1016/j.msec.2015.12.047
Open Access Status File (Author Post-print)
Volume 61
Start page 674
End page 680
Total pages 7
Place of publication Amsterdam, Netherlands
Publisher Elsevier BV
Language eng
Formatted abstract
Layered silicate nanoparticles (LSN) are widely used in industrial applications and consumer products. They also have potential benefits in biomedical applications such as implantable devices and for drug delivery. To study how nanomaterials interact with cells and tissues, techniques to track and quantify their movement through different biological compartments are essential. While radiolabels can be very sensitive, particularly for in vivo studies, fluorescent labeling has been preferred in recent years because of the array of methods available to image and quantify fluorescent nanoparticles. However, labeling can be problematic, especially if it alters the physical properties of the nanomaterial. Herein is described a novel non-covalent labeling technique for LSN using readily available fluorescent dimeric cyanine dyes without the need to use excess amounts of dye to achieve labeling, or the need for removal of unbound dye. The approach utilizes the cationic binding properties of layered silicate clays and the multiple quaternary nitrogens associated with the dyes. Preparation of YOYO-1 labeled LSN with optimal dispersion in aqueous media is presented. The utilization of the labeled particles is then demonstrated in cell binding and uptake studies using flow cytometry and confocal microscopy. The labeled LSN are highly fluorescent, stable and exhibit identical physical properties with respect to the unlabeled nanoparticles. The general approach described here is applicable to other cyanine dyes and may be utilized more widely for labeling nanoparticles that comprise a crystalline plate structure with a high binding capacity.
Keyword Fluorescence
Layered silicate nanoparticle non-covalent labeling
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

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