Methods for delivering DNA to intracellular receptors

Stacey, Katryn J., Idris, Adi, Sagulenko, Vitaliya, Vitak, Nazarii and Sester, David P. (2016). Methods for delivering DNA to intracellular receptors. In Toll-like receptor detection and activation 2nd ed. (pp. 93-106) New York, NY, United States: Springer Science+Business Media. doi:10.1007/978-1-4939-3335-8_6

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Author Stacey, Katryn J.
Idris, Adi
Sagulenko, Vitaliya
Vitak, Nazarii
Sester, David P.
Title of chapter Methods for delivering DNA to intracellular receptors
Title of book Toll-like receptor detection and activation
Place of Publication New York, NY, United States
Publisher Springer Science+Business Media
Publication Year 2016
Sub-type Research book chapter (original research)
DOI 10.1007/978-1-4939-3335-8_6
Open Access Status Not Open Access
Series Methods in Molecular Biology
Edition 2nd
ISBN 9781493933334
9781493933358
ISSN 1064-3745
Volume number 1390
Chapter number 6
Start page 93
End page 106
Total pages 14
Total chapters 25
Language eng
Formatted Abstract/Summary
Cytosolic DNA can indicate infection and induces type I interferon (IFN) and AIM2 inflammasome responses. Characterization of these responses has required introduction of DNA into the cytosol of macrophages by either chemical transfection or electroporation, each of which has advantages in different applications. We describe here optimized procedures for both electroporation and chemical transfection, including the centrifugation of chemical transfection reagent onto cells, which greatly increases the speed and strength of responses. Appropriate choice of DNA and use of these methods allow study of either the cytosolic DNA responses in isolation or the simultaneous stimulation of cytosolic receptors and the CpG DNA receptor toll-like receptor 9 (TLR9) in the endosomes.
Keyword AIM2
Centrifection
Cytosolic DNA
Electroporation
Inflammasome
Interferon
Macrophage
Pyroptosis
Transfection
cGAS
Q-Index Code B1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Book Chapter
Collections: HERDC Pre-Audit
School of Chemistry and Molecular Biosciences
 
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Created: Sat, 30 Jan 2016, 01:14:13 EST by Mrs Louise Nimwegen on behalf of School of Chemistry & Molecular Biosciences