Generation of footprint-free induced pluripotent stem cells from human fibroblasts using episomal plasmid vectors

Ovchinnikov, Dmitry A., Sun, Jane and Wolvetang, Ernst J. (2015) Generation of footprint-free induced pluripotent stem cells from human fibroblasts using episomal plasmid vectors. Methods in Molecular Biology, 1330 37-45. doi:10.1007/978-1-4939-2848-4_4

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Author Ovchinnikov, Dmitry A.
Sun, Jane
Wolvetang, Ernst J.
Title Generation of footprint-free induced pluripotent stem cells from human fibroblasts using episomal plasmid vectors
Journal name Methods in Molecular Biology   Check publisher's open access policy
ISSN 1064-3745
ISBN 9781493928484
9781493928477
Publication date 2015-12-15
Sub-type Article (original research)
DOI 10.1007/978-1-4939-2848-4_4
Open Access Status Not Open Access
Volume 1330
Start page 37
End page 45
Total pages 9
Place of publication New York, NY, United States
Publisher Humana Press
Language eng
Abstract Human induced pluripotent stem cells (hiPSCs) have provided novel insights into the etiology of disease and are set to transform regenerative medicine and drug screening over the next decade. The generation of human iPSCs free of a genetic footprint of the reprogramming process is crucial for the realization of these potential uses. Here we describe in detail the generation of human iPSC from control and disease-carrying individuals’ fibroblasts using episomal plasmids.
Keyword Human induced pluripotent stem cells
Reprogramming
Episomal plasmid vectors
Fibroblasts
Transfection
Genomic integration
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
Australian Institute for Bioengineering and Nanotechnology Publications
 
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Created: Wed, 27 Jan 2016, 18:51:30 EST by Dr Dmitry Ovchinnikov on behalf of Aust Institute for Bioengineering & Nanotechnology