Polyamines in prostatic epithelial cells and adenocarcinoma; the effects of androgen blockade

Cohen, Ronald J., Fujiwara, Kunio, Holland, John W. and McNeal, John E. (2001) Polyamines in prostatic epithelial cells and adenocarcinoma; the effects of androgen blockade. Prostate, 49 4: 278-284. doi:10.1002/pros.10023


Author Cohen, Ronald J.
Fujiwara, Kunio
Holland, John W.
McNeal, John E.
Title Polyamines in prostatic epithelial cells and adenocarcinoma; the effects of androgen blockade
Journal name Prostate   Check publisher's open access policy
ISSN 0270-4137
1097-0045
Publication date 2001-11-01
Sub-type Article (original research)
DOI 10.1002/pros.10023
Volume 49
Issue 4
Start page 278
End page 284
Total pages 7
Place of publication New York
Publisher Wiley-Liss
Language eng
Subject 110301 Anaesthesiology
11 Medical and Health Sciences
Formatted abstract
Background
Recent identification of eosinophilic prostatic secretory granules (PSG) as the major secretory mechanism of the prostate gland and their loss in neoplasia has prompted scrutiny of their chemical constituents. Polyamines, in particular spermine and spermidine (sp/spd) are the major cations found within prostatic secretions, yet their secretory mechanism in normal and neoplastic tissues has not been investigated.

Methods
Normal prostatic tissues and adenocarcinoma from intact and chemically castrated men were preserved in a glutaraldehyde-based fixative (Solufix®). Immunostains for sp/spd were performed before and after harsh acid hydrolysis whereby all protein was removed from tissue sections.

Results
Sp/spd immunoreactivity correlated with PSG as recognized in routine stains in tissues from intact patients before and after acid digestion. Decrease in sp/spd in untreated carcinomas was directly related to loss of PSG. After chemical castration, normal glands were mostly devoid of sp/spd while surviving malignant cells stained positively, despite a significant reduction or absence of PSG. Similarly, cancers progressing after castration were intensely decorated with anti-spermine, despite an almost complete loss of PSG. Cytoplasmic sp/spd staining of these androgen resistant clones was in contrast to normal glands no longer acid resistant.

Conclusions
The intense eosinophilia of PSG is attributable to polyamines. Androgen blockade arrests sp/spd production in normal tissue. In contrast, sp/spd production continues in androgen resistant tumor clones, thereby uncoupling polyamines from their normal androgen dependent environment. Prostate 49:278-284, 2001. © 2001 Wiley-Liss, Inc.
Keyword Endocrinology & Metabolism
Urology & Nephrology
Prostate Secretory Granule
Spermine
Spermidine
Anti-androgen
Rat Ventral Prostate
Identification
Spermine
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: Institute for Molecular Bioscience - Publications
 
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Citation counts: TR Web of Science Citation Count  Cited 15 times in Thomson Reuters Web of Science Article | Citations
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Created: Mon, 13 Aug 2007, 22:41:51 EST