Molecular comparison of bacterial communities on peripheral intravenous catheters and matched skin swabs

Choudhury, Md Abu, Marsh, Nicole, Banu, Shahera, Paterson, David L., Rickard, Claire M. and McMillan, David J. (2016) Molecular comparison of bacterial communities on peripheral intravenous catheters and matched skin swabs. PLoS ONE, 11 1: . doi:10.1371/journal.pone.0146354


Author Choudhury, Md Abu
Marsh, Nicole
Banu, Shahera
Paterson, David L.
Rickard, Claire M.
McMillan, David J.
Title Molecular comparison of bacterial communities on peripheral intravenous catheters and matched skin swabs
Journal name PLoS ONE   Check publisher's open access policy
ISSN 1932-6203
Publication date 2016-01-05
Sub-type Article (original research)
DOI 10.1371/journal.pone.0146354
Open Access Status DOI
Volume 11
Issue 1
Total pages 12
Place of publication San Francisco, United States
Publisher Public Library of Science
Language eng
Formatted abstract
Skin bacteria at peripheral intravenous catheter (PIVC) insertion sites pose a serious risk of microbial migration and subsequent colonisation of PIVCs, and the development of catheter related bloodstream infections (CRBSIs). Common skin bacteria are often associated with CRBSIs, therefore the bacterial communities at PIVC skin sites are likely to have major implications for PIVC colonisation. This study aimed to determine the bacterial community structures on skin at PIVC insertion sites and to compare the diversity with associated PIVCs. A total of 10 PIVC skin site swabs and matching PIVC tips were collected by a research nurse from 10 hospitalised medical/surgical patients at catheter removal. All swabs and PIVCs underwent traditional culture and high-throughput sequencing. The bacterial communities on PIVC skin swabs and matching PIVCs were diverse and significantly associated (correlation coefficient = 0.7, p<0.001). Methylobacterium spp. was the dominant genus in all PIVC tip samples, but not so for skin swabs. Sixty-one percent of all reads from the PIVC tips and 36% of all reads from the skin swabs belonged to this genus. Staphylococcus spp., (26%), Pseudomonas spp., (10%) and Acinetobacter spp. (10%) were detected from skin swabs but not from PIVC tips. Most skin associated bacteria commonly associated with CRBSIs were observed on skin sites, but not on PIVCs. Diverse bacterial communities were observed at skin sites despite skin decolonization at PIVC insertion. The positive association of skin and PIVC tip communities provides further evidence that skin is a major source of PIVC colonisation via bacterial migration but microbes present may be different to those traditionally identified via culture methods. The results provide new insights into the colonisation of catheters and potential pathogenesis of bacteria associated with CRBSI, and may assist in developing new strategies designed to reduce the risk of CRBSI.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: UQ Centre for Clinical Research Publications
HERDC Pre-Audit
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 0 times in Thomson Reuters Web of Science Article
Scopus Citation Count Cited 0 times in Scopus Article
Google Scholar Search Google Scholar
Created: Tue, 19 Jan 2016, 10:23:29 EST by System User on behalf of Learning and Research Services (UQ Library)