Ethnobotanical survey of Rinorea dentata (Violaceae) used in South-Western Nigerian ethnomedicine and detection of cyclotides

Attah, Alfred F., Hellinger, Roland, Sonibare, Mubo A., Moody, Jones O., Arrowsmith, Sarah, Wray, Susan and Gruber, Christian W. (2016) Ethnobotanical survey of Rinorea dentata (Violaceae) used in South-Western Nigerian ethnomedicine and detection of cyclotides. Journal of Ethnopharmacology, 179 83-91. doi:10.1016/j.jep.2015.12.038

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Author Attah, Alfred F.
Hellinger, Roland
Sonibare, Mubo A.
Moody, Jones O.
Arrowsmith, Sarah
Wray, Susan
Gruber, Christian W.
Title Ethnobotanical survey of Rinorea dentata (Violaceae) used in South-Western Nigerian ethnomedicine and detection of cyclotides
Formatted title
Ethnobotanical survey of Rinorea dentata (Violaceae) used in South-Western Nigerian ethnomedicine and detection of cyclotides
Journal name Journal of Ethnopharmacology   Check publisher's open access policy
ISSN 1872-7573
0378-8741
Publication date 2016-02-17
Sub-type Article (original research)
DOI 10.1016/j.jep.2015.12.038
Open Access Status File (Author Post-print)
Volume 179
Start page 83
End page 91
Total pages 9
Place of publication Shannon, Clare Ireland
Publisher Elsevier Ireland
Language eng
Subject 3004 Pharmacology
3002 Drug Discovery
Formatted abstract
Ethnopharmacological relevance

People living in the tropical rain forest of South-Western Nigeria use Rinorea dentata (P. Beauv.) Kuntze (Violaceae) in ethno-veterinary medicine to facilitate parturition. There are no evidence-based pharmacological investigations for the uterotonic activity of this plant.

Aims of study

(i) Collection of data about the ethnopharmacological uses of R. dentata and evaluation of its uses and applications in health care; (ii) determining potential uterotonic effects in vitro, and (iii) chemical characterization of R. dentata, which is a member of the Violaceae family known to express circular cystine-knot peptides, called cyclotides.

Materials and methods

The ethnopharmacological use of R. dentata in settlement camps within the area J4 of Omo forest has been investigated by semi-structured questionnaires and open interviews. Use index analysis has been performed by seven quantitative statistical models. Respondents’ claim on the beneficial ethno-veterinary application of the plant to aid parturition has been investigated in vitro by myometrial contractility organ bath assays. The bioactive plant extract was screened by chemical derivatization and mass spectrometry-based peptidomics using reversed-phase HPLC fractionation and MALDI-TOF/TOF analysis.

Results

Based on the survey analysis, medicinal preparations of R. dentata have been used for anti-microbial and anti-malaria purpose in humans, and for aiding parturition in farm animals. The latter application was mentioned by one out of six respondents who claimed to use this plant for any medicinal purpose. The plant extract exhibited a weak uterotonic effect using organ bath studies. The plant contains cyclotides and the peptide riden A has been identified by de novo amino acid sequencing using mass spectrometry.

Conclusion

Few dwellers around the settlement camps of the tropical forest of Omo (Nigeria) use R. dentata for various health problems in traditional veterinary and human medicine. The weak uterotonic effect of the cyclotide-rich extract is in agreement with the low use value index obtained for this plant. Cyclotides have been reported in the genus Rinorea confirming the ubiquitous expression of these stable bioactive plant peptides within the family of Violaceae.
Keyword Cyclotides
Ethnopharmacology
Rinorea dentata
Uterus muscle contractility
Veterinary medicine
Violaceae
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: HERDC Pre-Audit
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