Novel mouse model for simulating microsurgical tumor excision with facial nerve preservation

Lim, Jae H., Boyle, Glen M. and Panizza, Benedict (2016) Novel mouse model for simulating microsurgical tumor excision with facial nerve preservation. Laryngoscope, 126 1: E1-E5. doi:10.1002/lary.25545


Author Lim, Jae H.
Boyle, Glen M.
Panizza, Benedict
Title Novel mouse model for simulating microsurgical tumor excision with facial nerve preservation
Journal name Laryngoscope   Check publisher's open access policy
ISSN 0023-852X
1531-4995
Publication date 2016-01-01
Year available 2015
Sub-type Article (original research)
DOI 10.1002/lary.25545
Open Access Status Not Open Access
Volume 126
Issue 1
Start page E1
End page E5
Total pages 5
Place of publication Hoboken, NJ, United States
Publisher John Wiley & Sons
Language eng
Formatted abstract
Objectives/Hypothesis: To determine the feasibility of using a mouse tumor model as a microsurgical training tool for otolaryngology-head and neck surgery (OHNS) trainees.

Study Design: Animal study.

Methods:
We injected athymic nude mice with human cutaneous squamous cell carcinoma (A431 cell line) deep to the parotid region overlying the masseter muscle. We sacrificed the animals 1 to 3 weeks postinjection, once a visible tumor growth was confirmed. We then asked 10 OHNS trainees to excise the tumor with preservation of the facial nerves under a high-magnification dissecting microscope. The trainees graded the tasks in several areas of specific measures using a visual analogue scale (VAS) including 1) tumor texture, 2) surgical realism, 3) usefulness, and 4) difficulty of the task.

Results: Noticeable tumor growth occurred within 5 days following A431 cell injection and reached measureable size (0.5-1.5 cm) within 1 to 3 weeks. The tumor displaced the facial nerve laterally and medially, with few demonstrating infiltration of the nerve. VAS scores (± standard deviation) were 8.1 (±1.7), 7.7 (±2.5), 9.0 (±0.9) and 6.6 (±1.9) for tumor texture, surgical realism, usefulness, and the difficulty of the task, respectively.

Conclusions: We demonstrate a novel, reliable and cost-effective mouse model for simulating tumor extirpation microsurgery with preservation of important neural structures. OHNS trainees have found this simulation model to be realistic, useful, and appropriately challenging.
Keyword Microsurgery
Surgical simulation
Skull base tumor
Surgical training
Tumor model
Facial nerve
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Medicine Publications
 
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Created: Tue, 19 Jan 2016, 02:44:22 EST by Dr Benedict Panizza on behalf of Surgery - Princess Alexandra Hospital