The role of the paratrigeminal nucleus in vagal afferent evoked respiratory reflexes: A neuroanatomical and functional study in guinea pigs

Driessen, Alexandria K., Farrell, Michael J., Mazzone, Stuart B. and McGovern, Alice E. (2015) The role of the paratrigeminal nucleus in vagal afferent evoked respiratory reflexes: A neuroanatomical and functional study in guinea pigs. Frontiers in Physiology, 6 378: . doi:10.3389/fphys.2015.00378


Author Driessen, Alexandria K.
Farrell, Michael J.
Mazzone, Stuart B.
McGovern, Alice E.
Title The role of the paratrigeminal nucleus in vagal afferent evoked respiratory reflexes: A neuroanatomical and functional study in guinea pigs
Journal name Frontiers in Physiology   Check publisher's open access policy
ISSN 1664-042X
Publication date 2015-12-21
Year available 2015
Sub-type Article (original research)
DOI 10.3389/fphys.2015.00378
Open Access Status DOI
Volume 6
Issue 378
Total pages 11
Place of publication Lausanne, Switzerland
Publisher Frontiers Research Foundation
Language eng
Formatted abstract
The respiratory tree receives sensory innervation from the jugular and nodose vagal sensory ganglia. Neurons of these ganglia are derived from embryologically distinct origins and as such demonstrate differing molecular, neurochemical and physiological phenotypes. Furthermore, whereas nodose afferent neurons project to the nucleus of the solitary tract (nTS), recent neuroanatomical studies in rats suggest that jugular neurons have their central terminations in the paratrigeminal nucleus (Pa5). In the present study we confirm that guinea pigs demonstrate a comparable distinction between the brainstem terminations of nodose and jugular ganglia afferents. Thus, microinjection of fluorescently conjugated cholera toxin B (CT-B) neural tracers into the caudal nTS and Pa5 resulted in highly specific retrograde labeling of neurons in the nodose and jugular ganglia, respectively. Whereas, nodose neurons more often expressed 160 KD neurofilament proteins and the alpha3 subunit of Na+/K+ ATPase, significantly more jugular neurons expressed the neuropeptides substance P (SP) and, especially, Calcitonin Gene-Related Peptide (CGRP). Indeed, terminal fibers in the Pa5 compared to the nTS were characterized by their significantly greater expression of CGRP, further supporting the notion that jugular afferents project to trigeminal-related brainstem regions. Electrical stimulation of the guinea pig larynx following selective surgical denervation of the nodose afferent innervation to the larynx (leaving intact the jugular innervation) resulted in stimulus dependent respiratory slowing and eventual apnea. This jugular ganglia neuron mediated response was unaffected by bilateral microinjections of the GABAA agonist muscimol into the nTS, but was abolished by muscimol injected into the Pa5. Taken together these data confirm that jugular and nodose vagal ganglia afferent neurons innervate distinct central circuits and support the notion that multiple peripheral and central pathways mediate sensory responses associated with airway irritations.
Keyword Respiratory reflex
Vagal ganglia
Brainstem
Nucleus of the solitary tract
Airway afferents
Sensory innervation
Neuroanatomical tracing
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Official 2016 Collection
School of Biomedical Sciences Publications
 
Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 5 times in Thomson Reuters Web of Science Article | Citations
Scopus Citation Count Cited 4 times in Scopus Article | Citations
Google Scholar Search Google Scholar
Created: Sun, 17 Jan 2016, 10:19:44 EST by System User on behalf of Learning and Research Services (UQ Library)