Packed bed bioreactor for the isolation and expansion of placental-derived mesenchymal stromal cells

Osiecki, Michael J., Michl, Thomas D., Babur, Betul Kul, Kabiri, Mahboubeh, Atkinson, Kerry, Lott, William B., Griesser, Hans J. and Doran, Michael R. (2015) Packed bed bioreactor for the isolation and expansion of placental-derived mesenchymal stromal cells. PLoS One, 10 12: 1-18. doi:10.1371/journal.pone.0144941


Author Osiecki, Michael J.
Michl, Thomas D.
Babur, Betul Kul
Kabiri, Mahboubeh
Atkinson, Kerry
Lott, William B.
Griesser, Hans J.
Doran, Michael R.
Title Packed bed bioreactor for the isolation and expansion of placental-derived mesenchymal stromal cells
Journal name PLoS One   Check publisher's open access policy
ISSN 1932-6203
Publication date 2015-12-14
Year available 2015
Sub-type Article (original research)
DOI 10.1371/journal.pone.0144941
Open Access Status DOI
Volume 10
Issue 12
Start page 1
End page 18
Total pages 18
Place of publication San Francisco, CA United States
Publisher Public Library of Science
Language eng
Formatted abstract
Large numbers of Mesenchymal stem/stromal cells (MSCs) are required for clinical relevant doses to treat a number of diseases. To economically manufacture these MSCs, an automated bioreactor system will be required. Herein we describe the development of a scalable closed-system, packed bed bioreactor suitable for large-scale MSCs expansion. The packed bed was formed from fused polystyrene pellets that were air plasma treated to endow them with a surface chemistry similar to traditional tissue culture plastic. The packed bed was encased within a gas permeable shell to decouple the medium nutrient supply and gas exchange. This enabled a significant reduction in medium flow rates, thus reducing shear and even facilitating single pass medium exchange. The system was optimised in a small-scale bioreactor format (160 cm2) with murine-derived green fluorescent protein-expressing MSCs, and then scaled-up to a 2800 cm2 format. We demonstrated that placental derived MSCs could be isolated directly within the bioreactor and subsequently expanded. Our results demonstrate that the closed system large-scale packed bed bioreactor is an effective and scalable tool for large-scale isolation and expansion of MSCs.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Mater Research Institute-UQ (MRI-UQ)
UQ Centre for Clinical Research Publications
Official 2016 Collection
 
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