A live experimental vaccine against Burkholderia pseudomallei elicits CD4+ T cell-mediated immunity, priming T cells specific for 2 type III secretion system proteins

Haque, Ashraful, Chu, Karen, Easton, Anna, Stevens, Mark P., Galyov, Edouard E., Atkins, Tim, Titball, Rick and Bancroft, Gregory J. (2006) A live experimental vaccine against Burkholderia pseudomallei elicits CD4+ T cell-mediated immunity, priming T cells specific for 2 type III secretion system proteins. Journal of Infectious Diseases, 194 9: 1241-1248. doi:10.1086/508217


Author Haque, Ashraful
Chu, Karen
Easton, Anna
Stevens, Mark P.
Galyov, Edouard E.
Atkins, Tim
Titball, Rick
Bancroft, Gregory J.
Title A live experimental vaccine against Burkholderia pseudomallei elicits CD4+ T cell-mediated immunity, priming T cells specific for 2 type III secretion system proteins
Journal name Journal of Infectious Diseases   Check publisher's open access policy
ISSN 0022-1899
Publication date 2006-11-01
Year available 2006
Sub-type Article (original research)
DOI 10.1086/508217
Open Access Status Not yet assessed
Volume 194
Issue 9
Start page 1241
End page 1248
Total pages 8
Publisher UNIV CHICAGO PRESS
Language eng
Subject 2739 Public Health, Environmental and Occupational Health
2403 Immunology
Abstract Burkholderia pseudomallei is the etiological agent of melioidosis, a serious human disease for which no vaccine is available. Immunization of susceptible BALB/c mice with the live attenuated mutant B. pseudomallei ilvI (referred to as "2D2") generated significant, although incomplete, immunity. Splenic B. pseudomallei-specific T cells, detected in immunized mice, proliferated and produced interferon-γ in vitro in response to dead bacteria. Assessment of T cell antigen specificity indicated that subpopulations of B. pseudomallei-reactive T cells were responsive to BopE, a type III secretion system (TTSS) effector protein, and to a lesser extent to BipD, a TTSS translocator protein. Increased survival of severe combined immunodeficient mice adoptively transferred with T cells from immunized mice, compared with that of naive T cell recipients, demonstrated that immunization with 2D2 generated T cell-mediated immunity. CD4+ and CD8+ cell depletion studies demonstrated that CD4+ cells, but not CD8+ cells, mediated this protection in vivo. Thus, CD4+ T cells can mediate vaccine-induced immunity to experimental melioidosis.
Keyword Immunology
Infectious Diseases
Microbiology
Immunology
Infectious Diseases
Microbiology
IMMUNOLOGY
INFECTIOUS DISEASES
MICROBIOLOGY
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID BBS/E/I/00001116
AI-61363
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
 
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