Identification of a LolC homologue in Burkholderia pseudomallei, a novel protective antigen for melioidosis

Harland, David N., Chu, Karen, Haque, Ashraful, Nelson, Michelle, Walker, Nicola J., Sarkar-Tyson, Mitali, Atkins, Timothy P., Moore, Benjamin, Brown, Katherine A., Bancroft, Gregory, Titball, Richard W. and Atkins, Helen S. (2007) Identification of a LolC homologue in Burkholderia pseudomallei, a novel protective antigen for melioidosis. Infection and Immunity, 75 8: 4173-4180. doi:10.1128/IAI.00404-07

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Author Harland, David N.
Chu, Karen
Haque, Ashraful
Nelson, Michelle
Walker, Nicola J.
Sarkar-Tyson, Mitali
Atkins, Timothy P.
Moore, Benjamin
Brown, Katherine A.
Bancroft, Gregory
Titball, Richard W.
Atkins, Helen S.
Title Identification of a LolC homologue in Burkholderia pseudomallei, a novel protective antigen for melioidosis
Formatted title
Identification of a LolC homologue in Burkholderia pseudomallei, a novel protective antigen for melioidosis
Journal name Infection and Immunity   Check publisher's open access policy
ISSN 0019-9567
1098-5522
Publication date 2007-08-01
Sub-type Article (original research)
DOI 10.1128/IAI.00404-07
Open Access Status File (Publisher version)
Volume 75
Issue 8
Start page 4173
End page 4180
Total pages 8
Place of publication Washington, DC, United States
Publisher American Society for Microbiology
Language eng
Formatted abstract
Melioidosis is an emerging disease of humans in Southeast Asia and tropical Australia. The bacterium causing this disease, Burkholderia pseudomallei, is also considered a bioterrorism agent, and as yet there is no licensed vaccine for preventing B. pseudomallei infection. In this study, we evaluated selected proteins (LolC, PotF, and OppA) of the ATP-binding cassette systems of B. pseudomallei as candidate vaccine antigens. Nonmembrane regions of the B. pseudomallei proteins were expressed and purified from Escherichia coli and then evaluated as vaccine candidates in an established mouse model of B. pseudomallei infection. When delivered with the monophosphoryl lipid A-trehalose dicorynomycolate adjuvant, the proteins stimulated antigen-specific humoral and cellular immune responses. Immunization with LolC or PotF protein domains afforded significant protection against a subsequent challenge with B. pseudomallei. The most promising vaccine candidate, LolC, provided a greater level of protection when it was administered with immune-stimulating complexes complexed with CpG oligodeoxynucleotide 10103. Immunization with LolC also protected against a subsequent challenge with a heterologous strain of B. pseudomallei, demonstrating the potential utility of this protein as a vaccine antigen for melioidosis.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status Non-UQ

Document type: Journal Article
Sub-type: Article (original research)
Collection: School of Medicine Publications
 
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