Critical role of type 1 cytokines in controlling initial infection with Burkholderia mallei

Rowland, Caroline A., Lertmemongkolchai, Ganjana, Bancroft, Alison, Haque, Ashraful, Lever, M. Stephen, Griffin, Kate F., Jackson, Matthew C., Nelson, Michelle, O'Garra, Anne, Grencis, Richard, Bancroft, Gregory J. and Lukaszewski, Roman A. (2006) Critical role of type 1 cytokines in controlling initial infection with Burkholderia mallei. Infection and Immunity, 74 9: 5333-5340. doi:10.1128/IAI.02046-05

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Author Rowland, Caroline A.
Lertmemongkolchai, Ganjana
Bancroft, Alison
Haque, Ashraful
Lever, M. Stephen
Griffin, Kate F.
Jackson, Matthew C.
Nelson, Michelle
O'Garra, Anne
Grencis, Richard
Bancroft, Gregory J.
Lukaszewski, Roman A.
Title Critical role of type 1 cytokines in controlling initial infection with Burkholderia mallei
Journal name Infection and Immunity   Check publisher's open access policy
ISSN 0019-9567
Publication date 2006-01-01
Year available 2006
Sub-type Article (original research)
DOI 10.1128/IAI.02046-05
Open Access Status File (Publisher version)
Volume 74
Issue 9
Start page 5333
End page 5340
Total pages 8
Language eng
Subject 2403 Immunology
Abstract Burkholderia mallei is a gram-negative bacterium which causes the potentially fatal disease glanders in humans; however, there is little information concerning cell-mediated immunity to this pathogen. The role of gamma interferon (IFN-γ) during B. mallei infection was investigated using a disease model in which infected BALB/c mice normally die between 40 and 60 days postinfection. IFN-γ knockout mice infected with B. mallei died within 2 to 3 days after infection, and there was uncontrolled bacterial replication in several organs, demonstrating the essential role of IFN-γ in the innate immune response to this pathogen. Increased levels of IFN-γ, interleukin-6 (IL-6), and monocyte chemoattractant protein 1 were detected in the sera of immunocompetent mice in response to infection, and splenic mRNA expression of IFN-γ, IL-6, IL-12p35, and IL-27 was elevated 24 h postinfection. The effects of IL-18, IL-27, and IL-12 on stimulation of the rapid IFN-γ production were investigated in vitro by analyzing IFN-γ production in the presence of heat-killed B. mallei. IL-12 was essential for IFN-γ production in vitro; IL-18 was also involved in induction of IFN-γ, but IL-27 was not required for IFN-γ production in response to heat-killed B. mallei. The main cellular sources of IFN-γ were identified in vitro as NK cells, CD8+ T cells, and TCRγδ T cells. Our data show that B. mallei is susceptible to cell-mediated immune responses which promote expression of type 1 cytokines. This suggests that development of effective vaccines against glanders should target the production of IFN-γ. Copyright
Keyword Immunology
Infectious Diseases
Infectious Diseases
Q-Index Code C1
Q-Index Status Provisional Code
Grant ID MC_U117565642
Institutional Status Unknown

Document type: Journal Article
Sub-type: Article (original research)
Collection: ResearcherID Downloads - Archived
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