DGKθ Catalytic Activity Is Required for Efficient Recycling of Presynaptic Vesicles at Excitatory Synapses

Goldschmidt, Hana L., Tu-Sekine, Becky, Volk, Lenora, Anggono, Victor, Huganir, Richard L. and Raben, Daniel M. (2016) DGKθ Catalytic Activity Is Required for Efficient Recycling of Presynaptic Vesicles at Excitatory Synapses. Cell Reports, 14 2: 200-207. doi:10.1016/j.celrep.2015.12.022


Author Goldschmidt, Hana L.
Tu-Sekine, Becky
Volk, Lenora
Anggono, Victor
Huganir, Richard L.
Raben, Daniel M.
Title DGKθ Catalytic Activity Is Required for Efficient Recycling of Presynaptic Vesicles at Excitatory Synapses
Journal name Cell Reports   Check publisher's open access policy
ISSN 2211-1247
Publication date 2016-01-12
Year available 2015
Sub-type Article (original research)
DOI 10.1016/j.celrep.2015.12.022
Open Access Status DOI
Volume 14
Issue 2
Start page 200
End page 207
Total pages 8
Place of publication New York, United States
Publisher Elsevier
Language eng
Formatted abstract
Synaptic transmission relies on coordinated coupling of synaptic vesicle (SV) exocytosis and endocytosis. While much attention has focused on characterizing proteins involved in SV recycling, the roles of membrane lipids and their metabolism remain poorly understood. Diacylglycerol, a major signaling lipid produced at synapses during synaptic transmission, is regulated by diacylglycerol kinase (DGK). Here, we report a role for DGKθ in the mammalian CNS in facilitating recycling of presynaptic vesicles at excitatory synapses. Using synaptophysin- and vGlut1-pHluorin optical reporters, we found that acute and chronic deletion of DGKθ attenuated the recovery of SVs following neuronal stimulation. Rescue of recycling kinetics required DGKθ kinase activity. Our data establish a role for DGK catalytic activity at the presynaptic nerve terminal in SV recycling. Altogether, these data suggest that DGKθ supports synaptic transmission during periods of elevated neuronal activity.
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: Queensland Brain Institute Publications
Official 2016 Collection
 
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