Size-dependent gene delivery of amine-modified silica nanoparticles

Yu, Meihua, Niu, Yuting, Zhang, Jun, Zhang, Hongwei, Yang, Yannan, Taran, Elena, Jambhrunkar, Siddharth, Gu, Wenyi, Thorn, Peter and Yu, Chengzhong (2015) Size-dependent gene delivery of amine-modified silica nanoparticles. Nano Research, 9 2: 291-305. doi:10.1007/s12274-015-0909-5

Author Yu, Meihua
Niu, Yuting
Zhang, Jun
Zhang, Hongwei
Yang, Yannan
Taran, Elena
Jambhrunkar, Siddharth
Gu, Wenyi
Thorn, Peter
Yu, Chengzhong
Title Size-dependent gene delivery of amine-modified silica nanoparticles
Journal name Nano Research   Check publisher's open access policy
ISSN 1998-0000
Publication date 2015-12-28
Year available 2015
Sub-type Article (original research)
DOI 10.1007/s12274-015-0909-5
Open Access Status Not yet assessed
Volume 9
Issue 2
Start page 291
End page 305
Total pages 15
Place of publication Beijing, China
Publisher Tsinghua University Press
Language eng
Subject 2500 Materials Science
2208 Electrical and Electronic Engineering
Abstract Silica-based nanoparticles are promising carriers for gene delivery applications. To gain insights into the effect of particle size on gene transfection efficiency, amine-modified monodisperse Stöber spheres (NH2-SS) with diameters of 125, 230, 330, 440, and 570 nm were synthesized. The in vitro transfection efficiencies of NH2-SS for delivering plasmid DNA encoding green fluorescent protein (GFP) (pcDNA3-EGFP, abbreviated as pcDNA, 6.1 kbp) were studied in HEK293T cells. NH2-SS with a diameter of 330 nm (NH2-SS330) showed the highest GFP transfection level compared to NH2-SS particles with other sizes. The transfection efficiency was found as a compromise between the binding capacity and cellular uptake performance of NH2-SS330 and pcDNA conjugates. NH2-SS330 also demonstrated the highest transfection efficiency for plasmid DNA (pDNA) with a bigger size of 8.9 kbp. To our knowledge, this study is the first to demonstrate the significance of particle size for gene transfection efficiency in silica-based gene delivery systems. Our findings are crucial to the rational design of synthetic vectors for gene therapy.
Keyword Silica nanoparticles
Gene delivery
Plasmid DNA
Particle sizes
Cellular uptake
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

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