Size-dependent gene delivery of amine-modified silica nanoparticles

Yu, Meihua, Niu, Yuting, Zhang, Jun, Zhang, Hongwei, Yang, Yannan, Taran, Elena, Jambhrunkar, Siddharth, Gu, Wenyi, Thorn, Peter and Yu, Chengzhong (2015) Size-dependent gene delivery of amine-modified silica nanoparticles. Nano Research, 9 2: 291-305. doi:10.1007/s12274-015-0909-5

Author Yu, Meihua
Niu, Yuting
Zhang, Jun
Zhang, Hongwei
Yang, Yannan
Taran, Elena
Jambhrunkar, Siddharth
Gu, Wenyi
Thorn, Peter
Yu, Chengzhong
Title Size-dependent gene delivery of amine-modified silica nanoparticles
Journal name Nano Research   Check publisher's open access policy
ISSN 1998-0000
Publication date 2015-12-28
Year available 2015
Sub-type Article (original research)
DOI 10.1007/s12274-015-0909-5
Open Access Status Not yet assessed
Volume 9
Issue 2
Start page 291
End page 305
Total pages 15
Place of publication Beijing, China
Publisher Tsinghua University Press
Language eng
Abstract Silica-based nanoparticles are promising carriers for gene delivery applications. To gain insights into the effect of particle size on gene transfection efficiency, amine-modified monodisperse Stöber spheres (NH2-SS) with diameters of 125, 230, 330, 440, and 570 nm were synthesized. The in vitro transfection efficiencies of NH2-SS for delivering plasmid DNA encoding green fluorescent protein (GFP) (pcDNA3-EGFP, abbreviated as pcDNA, 6.1 kbp) were studied in HEK293T cells. NH2-SS with a diameter of 330 nm (NH2-SS330) showed the highest GFP transfection level compared to NH2-SS particles with other sizes. The transfection efficiency was found as a compromise between the binding capacity and cellular uptake performance of NH2-SS330 and pcDNA conjugates. NH2-SS330 also demonstrated the highest transfection efficiency for plasmid DNA (pDNA) with a bigger size of 8.9 kbp. To our knowledge, this study is the first to demonstrate the significance of particle size for gene transfection efficiency in silica-based gene delivery systems. Our findings are crucial to the rational design of synthetic vectors for gene therapy.
Keyword Silica nanoparticles
Gene delivery
Plasmid DNA
Particle sizes
Cellular uptake
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

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