Metagenome from a Spirulina digesting biogas reactor: Analysis via binning of contigs and classification of short reads

Nolla-Ardevol, Vimac, Peces, Miriam, Strous, Marc and Tegetmeyer, Halina E. (2015) Metagenome from a Spirulina digesting biogas reactor: Analysis via binning of contigs and classification of short reads. BMC Microbiology, 15 277: . doi:10.1186/s12866-015-0615-1


Author Nolla-Ardevol, Vimac
Peces, Miriam
Strous, Marc
Tegetmeyer, Halina E.
Title Metagenome from a Spirulina digesting biogas reactor: Analysis via binning of contigs and classification of short reads
Journal name BMC Microbiology   Check publisher's open access policy
ISSN 1471-2180
Publication date 2015-12-17
Year available 2015
Sub-type Article (original research)
DOI 10.1186/s12866-015-0615-1
Open Access Status DOI
Volume 15
Issue 277
Total pages 16
Place of publication London, United Kingdom
Publisher BioMed Central
Language eng
Formatted abstract
Background: Anaerobic digestion is a biological process in which a consortium of microorganisms transforms a complex substrate into methane and carbon dioxide. A good understanding of the interactions between the populations that form this consortium can contribute to a successful anaerobic digestion of the substrate. In this study we combine the analysis of the biogas production in a laboratory anaerobic digester fed with the microalgae Spirulina, a protein rich substrate, with the analysis of the metagenome of the consortium responsible for digestion, obtained by high-throughput DNA sequencing. The obtained metagenome was also compared with a metagenome from a full scale biogas plant fed with cellulose rich material.

Results: 
The optimal organic loading rate for the anaerobic digestion of Spirulina was determined to be 4.0 g Spirulina L−1 day−1 with a specific biogas production of 350 mL biogas g Spirulina −1 with a methane content of 68 %. Firmicutes dominated the microbial consortium at 38 % abundance followed by Bacteroidetes, Chloroflexi and Thermotogae. Euryarchaeota represented 3.5 % of the total abundance. The most abundant organism (14.9 %) was related to Tissierella, a bacterium known to use proteinaceous substrates for growth. Methanomicrobiales and Methanosarcinales dominated the archaeal community. Compared to the full scale cellulose-fed digesters, Pfam domains related to protein degradation were more frequently detected and Pfam domains related to cellulose degradation were less frequent in our sample.

Conclusions: 
The results presented in this study suggest that Spirulina is a suitable substrate for the production of biogas. The proteinaceous substrate appeared to have a selective impact on the bacterial community that performed anaerobic digestion. A direct influence of the substrate on the selection of specific methanogenic populations was not observed.
Keyword Metagenome
Metagenome comparison
Spirulina
Biogas
Binning
Microbial community
Q-Index Code C1
Q-Index Status Provisional Code
Institutional Status UQ

Document type: Journal Article
Sub-type: Article (original research)
Collections: School of Civil Engineering Publications
Official 2016 Collection
 
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